Beta-D-galactosidase and alpha-L-arabinofuranosidase activities during the softening of apples
Beta-D-galactosidase and alpha-L-arabinofuranosidase activities during the softening of apples
复制标题
苹果软化过程中的β-D-半乳糖苷酶和α-L-阿拉伯呋喃糖苷酶活性
DOI：
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发表时间：
1995
期刊：
影响因子：
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通讯作者：
Katsuyoshi Kaneko
中科院分区：
文献类型：
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作者：
H. Yoshioka;Y. Kashimura;Katsuyoshi Kaneko
从苹果（Mains dornestica Borkh.）细胞壁提取物中检测到4种β-D-半乳糖苷酶（GA-ase I、II、III和IV）和1种α-L-阿拉伯呋喃糖苷酶（AF-ase）活性。CV.通过羟基磷灰石柱层析分离的GA酶级分降解从苹果细胞壁制备的多糖苷酸，释放半乳糖。GA酶Ⅱ、Ⅲ、Ⅳ级分具有降解阿拉伯半乳聚糖和释放半乳糖的活性，而GA酶Ⅰ不能降解阿拉伯半乳聚糖。随着苹果贮藏软化，GA酶Ⅱ、Ⅲ、Ⅳ活性逐渐降低，而GA酶Ⅱ、Ⅲ、Ⅳ活性逐渐降低。在苹果采后贮藏期间，GA-ase I和AF-ase活性出现并增加，这些半乳糖和阿拉伯糖的释放活性可能与苹果软化过程中细胞壁中多聚糖醛酸苷、阿拉伯聚糖和半乳聚糖的降解和增溶有关。
Four β-D-galactosidases (GA-ase I, II, III, and IV) and one α-L-arabinofuranosidase (AF-ase) activities were detected in cell wall extracts from apples (Mains dornestica Borkh. cv. Starking Delicious) stored at 0°C for five months.The GA-ase fractions separated by hydroxylapatite column chromatography degraded a polyuronide prepared from apple cell walls, releasing galactose. GA-ase II, III, and IV fractions contained arabinogalactan degrading and galactose releasing activities, but GA- ase I failed to degrade arabinogalactan. AF-ase fraction degraded polyuronide and arabinogalactan releasing arabinose and other sugars.The activities of GA-ase II, III and IV decreased gradually as the apples softened in storage. The activities of GA-ase I and AF-ase which were not detectable at harvest, appeared and increased during storage.These galactose and arabinose releasing activities, may be involved in the degradation and solubilization of polyuronide, araban, and galactan in the cell walls of apples during softening.