Identification of specific gene expression profiles in human mast cells mediated by Toll-like receptor 4 and FcepsilonRI.
Identification of specific gene expression profiles in human mast cells mediated by Toll-like receptor 4 and FcepsilonRI.
复制标题
鉴定人肥大细胞中由 Toll 样受体 4 和 FcepsilonRI 介导的特定基因表达谱。
作者：
S. Okumura;J. Kashiwakura;H. Tomita;Kenji Matsumoto;T. Nakajima;H. Saito;Y. Okayama
啮齿类动物肥大细胞（mast cells，MCs）在先天性免疫和获得性免疫中起着重要作用。然而，迄今为止，没有证据表明人类MC参与先天免疫。我们发现，一个功能性的Toll样受体4（TLR 4）表达的人MCs时，它被上调干扰素γ（IFN-γ）。为了系统地探讨人类MCs如何调节TLR 4介导的激活和FcepsilonRI聚集后的免疫系统，我们使用高密度寡核苷酸探针阵列（GeneChip）比较脂多糖（LPS）诱导的基因表达谱与IgE/抗IgE介导的MCs谱。在MCs中观察到共享的核心反应和LPS或抗IgE特异性基因表达程序。此外，MCs表现出抗病毒反应基因程序响应IFN-γ，和LPS持续表达。与LPS刺激的人外周血单核细胞的基因表达谱相比，LPS刺激的MC特异性诱导包括Th 2细胞因子和趋化因子的基因子集，所述趋化因子招募Th 2细胞和嗜酸性粒细胞。这些结果表明，人类MCs表达定制的病原体和抗原特异性免疫反应，人类MCs可能在先天性和适应性免疫中发挥重要作用。
Rodent mast cells (MCs) are reported to play a pivotal role in both innate and adaptive immunity. However, there is so far no evidence that human MCs are involved in innate immunity. We found that a functional Toll-like receptor 4 (TLR4) was expressed on human MCs when it was up-regulated by interferon gamma (IFN-gamma). To systematically explore how human MCs modulate the immune system following TLR4-mediated activation and FcepsilonRI aggregation, we used high-density oligonucleotide probe arrays (GeneChip) to compare the lipopolysaccharide (LPS)-induced gene expression profile with the IgE/anti-IgE-mediated profile in MCs. Both a shared core response, and LPS- or anti-IgE-specific programs of gene expression were observed in MCs. Furthermore, MCs exhibited an antiviral response gene program in response to IFN-gamma, and LPS sustained that expression. Compared with the LPS-stimulated gene expression profile of human peripheral blood mononuclear cells, LPS-stimulated MCs specifically induced a subset of genes that included a Th2 cytokine and chemokines that recruit Th2 cells and eosinophils. These results reveal that human MCs express tailored pathogen- and antigen-specific immune responses and that human MCs may play important roles in innate and adaptive immunity.
影响因子：
30.5
作者：
Vladimir Toshchakov;Bryan W. Jones;P. Perera;K. Thomas;M. J. Cody;Shuling Zhang;B. Williams;J. Major;T. Hamilton;M. Fenton;S. Vogel
通讯作者：
Vladimir Toshchakov;Bryan W. Jones;P. Perera;K. Thomas;M. J. Cody;Shuling Zhang;B. Williams;J. Major;T. Hamilton;M. Fenton;S. Vogel
影响因子：
32.4
作者：
Doyle, SE;Vaidya, SA;Cheng, G
通讯作者：
Cheng, G
体外和体内衍生的小鼠肥大细胞上 Fc gamma RII 和 Fc gamma RIII 表达的成熟相关变化。
DOI：
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发表时间：
1990
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Katz,HR;Arm,JP;Benson,AC;Austen,KF
通讯作者：
Austen,KF