Identification of cryptochrome DASH from vertebrates
Identification of cryptochrome DASH from vertebrates
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DOI：
10.1111/j.1356-9597.2004.00738.x
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发表时间：
2004-05-01
期刊：
影响因子：
2.1
通讯作者：
Toh, H
中科院分区：
文献类型：
--
作者：
Daiyasu, H;Ishikawa, T;Toh, H
最近发现了一种新型隐花色素，即DASH。DASH蛋白是光裂合酶/隐花色素家族的第五个亚家族。已经从集胞藻属PCC 6803、霍乱弧菌和拟南芥中鉴定出了DASH-DASH。集胞藻DASH是第一个从细菌中鉴定出的隐花色素，其生化特征和三级结构已被广泛研究。为了确定该亚科在生物体内的分布范围，我们在几个数据库中搜索了新的DASH候选者。我们发现了五个序列作为新的DASH候选者，它们来自四种海洋细菌和粗糙脉孢菌。我们还从EST数据库中发现了许多DASH候选者，其中包括鱼类和两栖动物的序列。根据EST序列，我们克隆并测序了斑马鱼和非洲爪蟾候选人的cDNA。对这两个基因编码的蛋白质进行了纯化和表征。这两种蛋白都含有叶酸和黄素辅因子，并具有弱的DNA光裂合酶活性。系统发育分析表明，这7个候选基因实际上属于新型隐花色素亚家族。这是首次从脊椎动物和真菌中分离到DASH成员。
A new type of cryptochrome, CRY-DASH, has been recently identified. The CRY-DASH proteins constitute the fifth subfamily of the photolyase/cryptochrome family. CRY-DASHs have been identified from Synechocystis sp. PCC 6803, Vibrio cholerae, and Arabidopsis thaliana. The Synechocystis CRY-DASH was the first cryptochrome identified from bacteria, and its biochemical features and tertiary structure have been extensively investigated. To determine how broadly the subfamily is distributed within living organisms, we searched for new CRY-DASH candidates within several databases. We found five sequences as new CRY-DASH candidates, which are derived from four marine bacteria and Neurospora crassa. We also found many CRY-DASH candidates from the EST databases, which included sequences from fish and amphibians. We cloned and sequenced the cDNAs of the zebrafish and Xenopus laevis candidates, based on the EST sequences. The proteins encoded by the two genes were purified and characterized. Both proteins contained folate and flavin cofactors, and have a weak DNA photolyase activity. A phylogenetic analysis revealed that the seven candidates actually belong to the new type of cryptochrome subfamily. This is the first report of the CRY-DASH members from vertebrates and fungi.