Effect of lignin-derived lignophenols on vascular oxidative stress and inflammation in streptozotocin-induced diabetic rats
Effect of lignin-derived lignophenols on vascular oxidative stress and inflammation in streptozotocin-induced diabetic rats
复制标题
木质素衍生的木质酚对链脲佐菌素诱导的糖尿病大鼠血管氧化应激和炎症的影响
DOI：
10.1007/s11010-010-0645-9
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发表时间：
2011
影响因子：
4.3
通讯作者：
Sato S
中科院分区：
文献类型：
--
作者：
Mukai Y;Norikura T;Fujita S;Mikame K;Funaoka M;Sato S
天然木质素是植物界中丰富的有机聚合物，其衍生物是木素酚类化合物。本研究探讨LP是否能减轻链脲佐菌素（STZ）诱导的糖尿病大鼠血管氧化应激和炎症反应。将单次静脉注射链脲佐菌素（STZ）诱导的糖尿病大鼠随机分为两组，分别饲喂含0和1.0%脂蛋白的饲料。治疗5周后，超氧化物（O2-）的产生，烟酰胺腺嘌呤二核苷酸的mRNA表达水平（磷酸）（NAD（P）H）氧化酶亚基，单核细胞趋化蛋白-1（MCP-1）及其受体C-C趋化因子受体2（CCR 2），方法：采用免疫组织化学方法检测大鼠主动脉内皮细胞诱生型一氧化氮合酶（iNOS）的表达水平。注射对照和用或不用LP处理的糖尿病大鼠。在LP治疗的糖尿病大鼠主动脉中，发现增加的O2−产生和NAD（P）H氧化酶亚基Nox 4和p47 phox的mRNA表达水平显著降低。MCP-1和CCR 2的mRNA表达和iNOS的蛋白表达在未治疗的糖尿病大鼠的主动脉中被发现增加，而这些水平在LP治疗组中显著降低。这些结果表明，LP可以通过抑制NAD（P）H氧化酶减轻血管氧化应激和/或炎症。这可能导致糖尿病血管损害的改善。
Lignophenols (LP) are the derivatives of native lignin, which is an abundant organic polymer in the plant kingdom. This study investigated whether LP can attenuate vascular oxidative stress and inflammation in streptozotocin (STZ)-induced diabetic rats. The diabetic rats induced by a single intravenous injection of STZ were randomly divided into two groups fed either 0 or 1.0% LP-containing diet. After 5 weeks of treatment, the superoxide (O2−) production, mRNA expression levels of nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) oxidase subunits, monocyte chemoattractant protein-1 (MCP-1) and its receptor C–C chemokine receptor 2 (CCR2), and protein expression level of inducible nitric oxide synthase (iNOS) were examined in the aorta of vehicle-injected control and diabetic rats treated with or without LP. The increased O2−production and mRNA expression levels of NAD(P)H oxidase subunits Nox4 and p47phox were found to be significantly reduced in the aorta of diabetic rats treated with LP. The mRNA expression of MCP-1 and CCR2, and the protein expression of iNOS were found to be increased in the aorta of untreated diabetic rats, whereas these levels were significantly lower in the LP-treated group. These findings suggest that LP could attenuate vascular oxidative stress and/or inflammation via inhibition of NAD(P)H oxidase. This may lead to an improvement in the vascular impairment of diabetes.