Hydrogen sulfide interacting with abscisic acid in stomatal regulation responses to drought stress in Arabidopsis
Hydrogen sulfide interacting with abscisic acid in stomatal regulation responses to drought stress in Arabidopsis
复制标题
硫化氢与脱落酸相互作用对拟南芥干旱胁迫的气孔调节反应
DOI：
10.1016/j.plaphy.2012.10.017
复制
发表时间：
2013-01-01
影响因子：
6.5
通讯作者：
Pei, Yanxi
中科院分区：
文献类型：
--
作者：
Jin, Zhuping;Xue, Shaowu;Pei, Yanxi
硫化氢（H2S）在植物干旱胁迫下气孔关闭的调控中起着重要作用，而L-半胱氨酸脱氢酶（LCD）是半胱氨酸降解产生H2S的主要酶。鉴于H2S与脱落酸（阿坝）的相似作用，本研究以lcd、aba 3和abi 1突变体为研究对象，探讨H2S与阿坝之间的密切关系。lcd突变体比野生型植株气孔开度增大，对干旱胁迫更敏感。在lcd突变体中，Ca 2+通道和外向整流K+通道编码基因的表达降低，相反，内向整流K+通道编码基因的表达增加。aba 3和abi 1突变体的气孔开度在NaHS（H2S供体）处理后减小，但lcd突变体响应阿坝的气孔关闭受损。在aba 3和abi 1突变体中，LCD的表达和H2S产生速率均降低。阿坝受体候选物的转录表达在LCD突变体中上调，并且随着NaHS处理而降低。以上结果表明，H2S可能是阿坝通过离子通道调控气孔的重要环节，H2S影响阿坝受体候选物的表达，阿坝也影响H2S的产生。因此，H2S与阿坝在拟南芥干旱胁迫的气孔调节中相互作用。(c)2012年Elsevier Masson SAS。All rights reserved.
Hydrogen sulfide (H2S) plays a crucial role in the regulation of stomatal closure in plant response to drought stress, and L-cysteine desulfhydrase (LCD) has been identified as being mainly responsible for the degradation of cysteine to generate H2S. In view of the similar roles to abscisic acid (ABA), in this study, the lcd, aba3 and abi1 mutants were studied to investigate the close inter-relationship between H2S and ABA. The lcd mutant showed enlarged stomatal aperture and more sensitivity to drought stress than wild-type plants. Expression of Ca2+ channel and outward-rectifying K+ channel coding genes decreased in the lcd mutant, and conversely, expression of inward-rectifying K+ increased. The stomatal aperture of aba3 and abi1 mutants decreased after treatment with NaHS (a H2S donor), but stomatal closure in responses to ABA was impaired in the lcd mutant. The expression of LCD and H2S production rate decreased in both the aba3 and abi1 mutants. Transcriptional expression of ABA receptor candidates was upregulated in the lcd mutant and decreased with NaHS treatment. The above results suggested that H2S may be an important link in stomatal regulation by ABA via ion channels; H2S affected the expression of ABA receptor candidates; and ABA also influenced H2S production. Thus, H2S interacted with ABA in the stomatal regulation responsible for drought stress in Arabidopsis. (c) 2012 Elsevier Masson SAS. All rights reserved.