Comparative proteomic study and functional analysis of translationally controlled tumor protein in rice roots under Hg2+ stress
Comparative proteomic study and functional analysis of translationally controlled tumor protein in rice roots under Hg2+ stress
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Hg2胁迫下水稻根部翻译控制肿瘤蛋白的比较蛋白质组学研究和功能分析
DOI：
10.1016/s1001-0742(11)61062-0
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发表时间：
2012-01-01
影响因子：
6.9
通讯作者：
Zhu, Cheng
中科院分区：
文献类型：
--
作者：
Wang, Feijuan;Shang, Yongshen;Zhu, Cheng
到目前为止，在蛋白质组水平上对汞胁迫诱导水稻根细胞间代谢变化的了解甚少。为了研究水稻根系对汞胁迫的响应，采用比较蛋白质组学方法分析了水稻根系中蛋白质表达的变化。以50 μ mol/L的HgCl2处理6叶期水稻幼苗3小时；与耐Hg2+突变型和野生型（中华11号）相比，胁迫下29个蛋白斑点的丰度发生了显著变化。此外，所有这些蛋白质点都被质谱鉴定为27种不同的蛋白质物种。所鉴定的蛋白质参与了应激反应、氧化还原稳态、信号转导、调节和代谢等过程；其中一些被发现是细胞结构蛋白，还有一些是未知的。在上调的蛋白中，我们选择了OsTCTP（翻译控制的肿瘤蛋白）在酵母中进行了外源表达，推测其参与了水稻对Hg2+的耐受，为其减轻Hg2+损伤的作用提供了证据。在众多试验中，我们发现ostctp过表达酵母菌株比野生型酵母菌对Hg2+的抗性更强。因此，我们认为OsTCTP有助于Hg2+抗性。本文首次报道了植物中与Hg2+胁迫有关的OsTCTP的功能特征。
So far, very little is known about mercury stress-induced intercellular metabolic changes in rice roots at the proteome level. To investigate the response of rice roots to mercury stress, changes in protein expression in rice roots were analyzed using a comparative proteomics approach. Six-leaf stage rice seedlings were treated with 50 mu mol/L HgCl2 for 3 hr; 29 protein spots showed a significant changes in abundance under stress when compared with the Hg2+-tolerant rice mutant and wild type (Zhonghua 11). Furthermore, all these protein spots were identified by mass spectrometry to match 27 diverse protein species. The identified proteins were involved in several processes, including stress response, redox homeostasis, signal transduction, regulation and metabolism; some were found to be cellular structure proteins and a few were unknown. Among the up-regulated proteins, OsTCTP (translationally controlled tumor protein) was chosen to perform hetereologous expression in yeast which was presumed to participate in the Hg2+ tolerance of rice, providing evidence for its role in alleviating Hg2+ damage. Among the many tests, we found that OsTCTP-overexpressed yeast strains were more resistant to Hg2+ than wild-type yeast. Thus, we propose that OsTCTP contributes to Hg2+ resistance. Here we present, for the first time, the functional characterization of OsTCTP in connection with Hg2+ stress in plants.