In Vitro and In Vivo Anticancer Activity of Human β-Defensin-3 and Its Mouse Homolog
In Vitro and In Vivo Anticancer Activity of Human β-Defensin-3 and Its Mouse Homolog
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DOI：
10.21873/anticanres.11188
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发表时间：
2016-11-01
影响因子：
2
通讯作者：
Ouchi, Yasuyoshi
中科院分区：
文献类型：
--
作者：
Hanaoka, Yoko;Yamaguchi, Yasuhiro;Ouchi, Yasuyoshi
背景/目的：防御素是哺乳动物阳离子抗菌肽家族的成员。我们研究了人β-防御素-3（hBD 3）及其小鼠同源物Defb 14对肺癌细胞的抗癌作用。材料和方法：我们用碘化丙啶和Hoechst 33342对用防御素肽处理后培养的肺癌细胞进行染色。在体内，在小鼠皮下刘易斯肺癌细胞肿瘤附近连续输注Defb 14肽或载体。输注9天后，测定切除肿瘤的重量。结果：用hBD 3（70 μ g/ml）处理10分钟诱导肺癌细胞摄取碘化丙啶。hBD 3的抗癌活性显著强于其他防御素亚型的活性。连续输注Defb 14肽在小鼠中的刘易斯肺癌细胞中显示出显著的肿瘤生长抑制。结论：我们的研究证实了Defb 14肽在动物模型中抑制肿瘤生长。
Background/Aim: Defensins comprise a family of mammalian cationic antimicrobial peptides. We investigated the anticancer effects of human beta-defensin-3 (hBD3) and its mouse homolog, Defb14, on lung cancer cells. Materials and Methods: We stained lung cancer cells cultured after treatment with the defensin peptide using propidium iodide and Hoechst 33342. In vivo, Defb14 peptide or vehicle was continuously infused near subcutaneous Lewis lung carcinoma cell tumor in mice. After 9-day infusion, the weights of excised tumors were determined. Results: A 10-min treatment with hBD3 (70 mu g/ml) induced propidium iodide uptake in lung cancer cells. The anticancer activity of hBD3 was significantly more potent than the activity of other defensin isoforms. Continuous infusion of Defb14 peptide showed significant tumor-growth suppression in Lewis lung carcinoma cells in mice. Conclusion: Our study demonstrated the suppression of tumor growth by Defb14 peptide in an animal model.