Rapid repair of titanium particle-induced osteolysis is dramatically reduced in aged mice
Rapid repair of titanium particle-induced osteolysis is dramatically reduced in aged mice
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DOI：
10.1016/s0736-0266(00)00033-4
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发表时间：
2001-03-01
影响因子：
2.8
通讯作者：
Greenfield, EM
中科院分区：
文献类型：
--
作者：
Kaar, SG;Ragab, AA;Greenfield, EM
无菌性松动是骨科假体失败的最常见原因。这一过程被认为是由植入物产生的磨损颗粒引起的骨溶解。Teitelbaum及其同事最近开发了一种很有前途的小鼠颅骨模型，用于磨损颗粒诱导的骨溶解。然而，在本研究之前，该模型仅进行了定性评估。我们现在报告了一个可重复的，定量版本的磨损颗粒诱导的骨溶解颅骨模型，其中整个顶骨的骨溶解（和修复）的程度是通过接触显微x线摄影的组织形态学来评估的。使用该模型，我们发现在一个月大的小鼠中，溶骨反应是短暂的，并迅速修复。骨溶解程度在颗粒植入后7天达到峰值，并在13天恢复到基线水平。当颗粒反复植入时，观察到类似数量的骨溶解，甚至更广泛的修复。相反，老年小鼠出现进行性骨溶解，没有可检测到的修复。结果，植入颗粒21天后，26月龄小鼠的骨溶解率是1月龄小鼠的17倍左右，骨骼成熟的成年小鼠（4-16月龄）表现出中等程度的反应。这些小鼠的骨溶解达到峰值。粒子植入后7天，但修复速度比1月龄小鼠慢。综上所述;这些结果强调了骨吸收和骨形成之间的不平衡在无菌性松动发展中的作用，并提示刺激骨形成的药物可能有助于预防或治疗无菌性松动。(C) 2001骨科研究学会。Elsevier Science Ltd.出版。版权所有。
Aseptic loosening is the most common cause of orthopaedic implant failure. This process is thought to be due to osteolysis induced by implant-derived wear particles. Teitelbaum and colleagues have recently developed a promising murine calvarial model of wear particle-induced osteolysis. However, prior to this study, this model had only been assessed qualitatively. We now report a reproducible, quantitative version of the calvarial model of wear particle-induced osteolysis, in which the extent of osteolysis (and repair) of entire parietal bones is assessed by histomorphometry of contact microradiographs. Using this model, we found that the osteolytic response is transient and rapidly repaired in one month old mice. The extent of osteolysis peaks 7 days after particle implantation and returns to baseline levels by 13 days. A similar amount of osteolysis and even more extensive repair is observed when particles are implanted repeatedly. In contrast, aged mice develop progressive osteolysis with no detectable repair. As a result, 26 month old mice have approximately 17-fold more osteolysis than one month old mice 21 days after particle implantation, Skeletally mature, adult mice (4-16 months old) show an intermediate pattern of response. Osteolysis in these mice peaks. at 7 days after particle implantation but it is repaired more slowly than ill the one month old mice. Taken together; these results underscore the role of an imbalance between bone resorption and bone formation in the development of aseptic loosening and suggest that agents that stimulate bone formation maybe useful in prevention ol treatment of aseptic loosening. (C) 2001 Orthopaedic Research Society. Published by Elsevier Science Ltd. All rights reserved.