CIPK7 is involved in cold response by interacting with CBL1 in Arabidopsis thaliana
CIPK7 is involved in cold response by interacting with CBL1 in Arabidopsis thaliana
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CIPK7 通过与拟南芥 CBL1 相互作用参与冷反应
DOI：
10.1016/j.plantsci.2011.03.011
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发表时间：
2011-07-01
期刊：
影响因子：
5.2
通讯作者：
An, Lizhe
中科院分区：
文献类型：
--
作者：
Huang, Conglin;Ding, Shuo;An, Lizhe
类钙调神经磷酸酶B（calcineurin B-like，CBL）蛋白家族是植物中一类独特的钙离子感受器。CBL通过与CBL相互作用蛋白激酶（CIPK）家族相互作用并调节CIPK家族来传递钙信号。大量的研究表明CBL-CIPK复合物介导植物对各种外部胁迫的响应。然而，目前关于CBL-CIPK参与冷应激反应的研究报道较少。在这项研究中，我们分析了CIPK 7和CBL 1在拟南芥在冷处理过程中的表达。CIPK 7在体外与CBL 1相互作用。此外，使用CBL 1从拟南芥植物中亲和层析纯化CIPK 7表明CIPK 7可能与CBL 1在体内缔合。CBL 1的表达是冷诱导的，CBL 1在调节冷反应中起作用。通过比较CIPK 7在野生型和cbl 1突变体植物中的表达模式，我们发现CIPK 7在冷胁迫下的诱导受到CBL 1的影响。这是第一份证明CIPK 7可能通过与CBL 1相互作用在冷反应中发挥作用的报告。(C)2011爱思唯尔爱尔兰有限公司保留所有权利。
The family of calcineurin B-like (CBL) proteins is a unique group of Ca(2+) sensors in plants. CBLs relay the calcium signal by interacting with and regulating the family of CBL-interacting protein kinases (CIPKs). Extensive studies have demonstrated that the CBL-CIPK complexes mediate plant responses to a variety of external stresses. However, there are few reports on the CBL-CIPK involved in cold stress responses. In this study, we analyzed expression of CIPK7 and CBL1 in Arabidopsis during cold treatments. Expression of CIPK7 was induced by cold, and CIPK7 interacted with CBL1 in vitro. Moreover, affinity chromatography purification of CIPK7 from Arabidopsis plants using CBL1 suggested that CIPK7 may associate with CBL1 in vivo. Expression of CBL1 was cold inducible, and CBL1 had a role in regulating cold response. By comparing expression patterns of CIPK7 between wild-type and cbl1 mutant plants, we found the induction of CIPK7 by cold stress was influenced by CBL1. This is the first report to demonstrate that CIPK7 may play a role in cold response via its interaction with CBL1. (C) 2011 Elsevier Ireland Ltd. All rights reserved.