Making patch-pipettes and sharp electrodes with a programmable puller.
Making patch-pipettes and sharp electrodes with a programmable puller.
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DOI：
10.3791/939
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发表时间：
2008-10-08
期刊：
影响因子：
--
通讯作者：
Goodman, Miriam B
中科院分区：
文献类型：
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作者：
Brown, Austin L;Johnson, Brandon E;Goodman, Miriam B
Glass microelectrodes (also called pipettes) have been a workhorse of electrophysiology for decades. Today, such pipettes are made from glass capillaries using a programmable puller. Such instruments heat the capillary using either a metal filament or a laser and draw out the glass using gravity, a motor or both. Pipettes for patch-clamp recording are formed using only heat and gravity, while sharp electrodes for intracellular recording use a combination of heat, gravity, and a motor. The procedure used to make intracellular recording pipettes is similar to that used to make injection needles for a variety of applications, including cRNA injection into Xenopus oocytes. In general, capillary glass
