Characterization of the mouse hnRNP A2/B1/B0 gene and identification of processed pseudogenes
Characterization of the mouse hnRNP A2/B1/B0 gene and identification of processed pseudogenes
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DOI：
10.1016/s0378-1119(02)00800-4
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发表时间：
2002-07-24
期刊：
影响因子：
3.5
通讯作者：
Smith, R
中科院分区：
文献类型：
--
作者：
Hatfield, JT;Rothnagel, JA;Smith, R
已使用基于PCR的策略克隆小鼠hnRNP A2/B1/B 0基因并测序。对该序列的分析表明，该基因的组织结构与人类直向同源物的组织结构密切相关，具有12个外显子和11个内含子。hnRNP A2/B1/B 0基因通过外显子2和9的选择性剪接产生四种剪接变体。RT-PCR分析表明，所有的剪接变异体表达在不同年龄的小鼠脑，皮肤和胃组织，虽然他们的比例与另一个不同的年龄和组织类型。我们还确定了一个小子集的所有多聚腺苷酸化剪接变异体，包括内含子11，这表明94%的序列之间的人类和小鼠的一致性。在小鼠基因组中发现了几个经过加工的假基因。对小鼠基因组数据库的搜索找到了五个假基因，其中四个是。由于在编码区内存在提前终止密码子、大的缺失或重排，推测其是非功能性的。第五，它拥有推定的启动子元件，并具有相同的编码序列的hnRNPA 2 mRNA，变体，可能是功能性的。(C)2002 Elsevier Science B. V.保留所有权利。
The mouse hnRNP A2/B1/B0 gene has been cloned using a PCR-based strategy and sequenced. Analysis of this sequence showed that the gene organization closely follows that of the human orthologue with 12 exons and 11 introns. The hnRNP A2/B1/B0 gene gives rise to four splice variants through alternative splicing of exons 2 and 9. RT-PCR assays indicated that all splice variants were expressed in mouse brain, skin, and stomach tissues of varying ages, although their ratios to one another varied with age and tissue type. We also identified a small subset of all polyadenylated splice variants that included intron 11, which shows 94% sequence identity between human and mouse. Several processed pseudogenes were identified in the mouse genome. A search of the mouse genome databases located five pseudogenes, four of. which are presumed to be non-functional because of the presence of premature stop codons, large deletions or rearrangements within the coding region. The fifth, which possesses putative promoter elements and has a coding sequence identical to that of the hnRNP A2 mRNA, variant, may be functional. (C) 2002 Elsevier Science B.V. All rights reserved.