Selective Release of Multiple DNA Oligonucleotides from Gold Nanorods
Selective Release of Multiple DNA Oligonucleotides from Gold Nanorods
复制标题
DOI：
10.1021/nn800702n
复制
发表时间：
2009-01-01
期刊：
影响因子：
17.1
通讯作者：
Hamad-Schifferli, Kimberly
中科院分区：
文献类型：
--
作者：
Wijaya, Andy;Schaffer, Stefan B.;Hamad-Schifferli, Kimberly
联合治疗或使用多种药物已被证明对复杂疾病有效，但化学性质和药代动力学的差异可能在装载，递送和释放多种药物方面具有挑战性。在这里，我们证明了我们可以从两种不同的金纳米棒中加载并选择性地释放两种不同的DNA寡核苷酸。通过巯基缀合将DNA负载在纳米棒上。通过在纳米棒的纵向表面等离子体共振峰处的超快激光照射，通过选择性熔化金纳米棒来诱导选择性释放。一种波长的激发可以选择性地熔化一种类型的金纳米棒，并选择性地释放一种类型的DNA链。释放是有效的（50-80%）和外部可调的激光通量。释放的寡核苷酸仍然具有功能。这种概念验证可能是多药物递送策略的有力方法。
Combination therapy, or the use of multiple drugs, has been proven to be effective for complex diseases, but the differences in chemical properties and pharmacokinetics can be challenging in terms of the loading, delivering, and releasing multiple drugs. Here we demonstrate that we can load and selectively release two different DNA oligonucleotides from two different gold nanorods. DNA was loaded on the nanorods via thiol conjugation. Selective releases were induced by selective melting of gold nanorods via ultrafast laser irradiation at the nanorods' longitudinal surface plasmon resonance peaks. Excitation at one wavelength could selectively melt one type of gold nanorods and selectively release one type of DNA strand. Releases were efficient (50-80%) and externally tunable by laser fluence. Released oligonucleotides were still functional. This proof of concept is potentially a powerful method for multiple-drug delivery strategies.