Haemophilus influenzae disease in the White Mountain Apaches: molecular epidemiology of a high risk population.
Haemophilus influenzae disease in the White Mountain Apaches: molecular epidemiology of a high risk population.
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白山阿帕奇人流感嗜血杆菌病：高危人群的分子流行病学。
DOI：
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发表时间：
1984
期刊：
影响因子：
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通讯作者：
E. Moxon
中科院分区：
文献类型：
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作者：
G. Losonsky;M. Santosham;Vija Sehgal;A. Zwahlen;E. Moxon
根据 10 年回顾和 15 个月前瞻性分析，白山阿帕奇印第安人 5 岁以下儿童的流感嗜血杆菌脑膜炎年发病率为 254 例/10 万，比美国普通人群的发病率高 8 倍。在我们的前瞻性监测中，从全身性疾病病例中分离出的流感嗜血杆菌进一步通过荚膜类型、生物型以及十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）上的外膜蛋白（OMP）和脂多糖（LPS）模式进行表征。出乎意料的是，在3/18的全身性疾病病例中分离出a型流感嗜血杆菌菌株。所有其他致病菌株均为 b 型。具有共同 OMP、LPS、荚膜和生物型特征的 b 型流感嗜血杆菌菌株是从保护区内不同地理群落的个体中分离出来的。这些表面上相同的菌株是从不同的个体中分离出来的，这些个体的系统性感染发生时间相隔数月（最长间隔为 14 个月）。 OMP 和 LPS 亚型对于区分侵入性流感嗜血杆菌分离株都是必要的。
Based on a 10-year retrospective and a 15-month prospective analysis, White Mountain Apache Indian children younger than 5 years were found to have an annual incidence of Haemophilus influenzae meningitis of 254 cases/100,000--an 8-fold higher incidence than that seen in the general United States population. In our prospective surveillance, H. influenzae isolates from cases of systemic disease were further characterized by capsular type, biotype and by outer membrane protein (OMP) and lipopolysaccharide (LPS) patterns on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Unexpectedly, H. influenzae type a strains were isolated in 3/18 cases of systemic disease. All other disease-producing strains were type b. H. influenzae b strains possessing common OMP, LPS, capsular and biotype characteristics were isolated from individuals in geographically distinct communities within the reservation. These apparently identical strains were isolated from different individuals who had systemic infections that occurred months apart (longest interval, 14 months). Both OMP and LPS subtyping were necessary to discriminate among invasive H. influenzae isolates.