Identification and Characterization of Lysobacter enzymogenes as a Biological Control Agent Against Some Fungal Pathogens
Identification and Characterization of Lysobacter enzymogenes as a Biological Control Agent Against Some Fungal Pathogens
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DOI：
10.1016/s1671-2927(09)60010-9
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发表时间：
2009-01-01
影响因子：
--
通讯作者：
Liu Feng-quan
中科院分区：
文献类型：
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作者：
Qian Guo-liang;Hu Bai-shi;Liu Feng-quan
从江苏省农业科学院绿色辣椒根际分离到一株革兰氏阴性、不产芽孢、具有较强拮抗活性的杆状细菌OH 11。16 S rRNA基因序列分析表明，菌株OH 11属于γ-变形菌门，与产酶溶杆菌菌株C3的序列相似性最高（AY 074793）（99%）、产酶溶杆菌菌株N4-7（U89965）（99%）、嗜热溶杆菌菌株（AB 019582）（97%）和胶溶杆菌菌株（AB 16136）（97%）。化学分类学数据显示菌株OH 11具有奎宁系统，其中Q-8为主要化合物，C-15：0 iso，C-17：1 iso omega 9 c为主要的异支链脂肪酸，所有这些都证实菌株OH 11归属于溶杆菌属。DNA-DNA杂交和生理生化试验结果表明，菌株OH 11属于产酶溶杆菌（Lysobacter enzymogenes）。菌株OH 11能产生蛋白酶、几丁质酶和β-1，3-葡聚糖酶。该化合物对水稻纹枯病菌、核盘菌等植物病原真菌具有较强的体外抑菌活性。这是国内首次报道产酶溶杆菌作为植物病害生防菌的鉴定和特性。
Strain OH11, a Gram-negative, nonspore forming, rod-shaped bacterium with powerful antagonistic activity, was isolated from rhizosphere of green pepper in Jiangsu Academy of Agricultural Sciences of China and characterized to determine its taxonomic position. 16S rRNA gene sequence analysis revealed that strain OH11 belongs to the Gammaproteobacteria and had the highest degree of sequence similarity to Lysobacter enzymogenes strain C3 (AY074793) (99%), Lysobacter enzymogenes strain N4-7 (U89965) (99%), Lysobacter antibioticus strain (AB019582) (97%), and Lysobacter gummosus strain (AB16136) (97%). Chemotaxonomic data revealed that strain OH11 possesses a quinine system with Q-8 as the predominant compound and C-15:0 iso, C-17:1 iso omega 9c as the predominant iso-branched fatty acids, all of which corroborated the assignment of strain OH11 to the genus Lysobacter. Results of DNA-DNA hybridization and physiological and biochemical tests clearly showed that strain OH11 was classified as Lysobacter enzymogenes. Strain OH11 could produce protease, chitinase, and beta-1,3-glucanase. It showed strong in vitro antifungal activity against Rhizoctonia solani, Sclerotinia scletotiorum, and several other phytopathogenic fungi. This is the first report of identification and characterization of Lysobacter enzymogenes as a biological control agent of plant diseases in China.