The small GTPase RhoG regulates microtubule-mediated focal adhesion disassembly.
The small GTPase RhoG regulates microtubule-mediated focal adhesion disassembly.
复制标题
小 GTP 酶 RhoG 调节微管介导的粘着斑分解。
DOI：
10.1038/s41598-019-41558-7
复制
发表时间：
2019
影响因子：
4.6
通讯作者：
Garcia-Mata,Rafael
中科院分区：
文献类型：
--
作者：
Zinn,Ashtyn;Goicoechea,SilviaM;Kreider-Letterman,Gabriel;Maity,Debonil;Awadia,Sahezeel;Cedeno-Rosario,Luis;Chen,Yun;Garcia-Mata,Rafael
Focal adhesions (FA) are a complex network of proteins that allow the cell to form physical contacts with the extracellular matrix (ECM). FA assemble and disassemble in a dynamic process, orchestrated by a variety of cellular components. However, the underlying mechanisms that regulate adhesion turnover remain poorly understood. Here we show that RhoG, a Rho GTPase related to Rac, modulates FA dynamics. When RhoG expression is silenced, FA are more stable and live longer, resulting in an increase in the number and size of adhesions, which are also more mature and fibrillar-like. Silencing RhoG also increases the number and thickness of stress fibers, which are sensitive to blebbistatin, suggesting contractility is increased. The molecular mechanism by which RhoG regulates adhesion turnover is yet to be characterized, but our results demonstrate that RhoG plays a role in the regulation of microtubule-mediated FA disassembly.
