Immortalization of BGDF (BCGF II)- and BCDF-producing T cells by human T cell leukemia virus (HTLV) and characterization of human BGDF (BCGF II).
Immortalization of BGDF (BCGF II)- and BCDF-producing T cells by human T cell leukemia virus (HTLV) and characterization of human BGDF (BCGF II).
复制标题
人 T 细胞白血病病毒 (HTLV) 对产生 BGDF (BCGF II) 和 BCDF 的 T 细胞进行永生化，并对人 BGDF (BCGF II) 进行表征。
作者：
K. Shimizu;T. Hirano;K. Ishibashi;N. Nakano;T. Taga;K. Sugamura;Y. Yamamura;T. Kishimoto
用人T细胞白血病病毒（HTLV）转化人外周血T细胞，建立了产生BGDF（BCGF II）和BCDF的T细胞系。在这些细胞系中，细胞系TCL-Na 1分泌最高水平的BGDF和BCDF，并且由TCL-Na 1细胞分泌的BCDF的量是由PHA刺激的T细胞产生的BCDF的量的900倍。在我们的检查范围内，没有HTLV转化的T细胞系产生IL 2或BSF-β 1（BCGF I）。由TCL-Na 1细胞产生的BCDF的分子量为1000 μ g/L。35，000和pI值为5.5，与BGDF分离，BGDF在对应于m.w.大于60，000，pI值为5至6。BGDF在小鼠白血病B细胞系BCL 1中诱导增殖和IgM分泌，并且在存在或不存在0.1% Triton X-100的情况下，这些活性未通过等电聚焦或凝胶过滤分离，表明命名为BGDF的分子具有生长和分化活性。BGDF作用于正常小鼠B细胞以诱导增殖以及IgM分泌。BGDF的靶细胞是体内活化的B母细胞。BGDF作用于DXS激活的小鼠B细胞，诱导增殖和IgM分泌，但不诱导抗Ig激活的B细胞，表明BGDF和BSF-p1是不同的分子。
Human peripheral T cells were transformed by human T cell leukemia virus (HTLV), and T cell lines producing BGDF (BCGF II) and BCDF were established. Among these cell lines, a cell line, TCL-Na1, secreted the highest level of both BGDF and BCDF, and the amount of BCDF secreted by TCL-Na1 cells was 900-fold more than that produced by PHA-stimulated T cells. Within the limits of our examination, none of the HTLV-transformed T cell lines produced IL 2 or BSF-p1 (BCGF I). BCDF produced by TCL-Na1 cells had a m.w. of 35,000 and a pI value of 5.5, being separated from BGDF, which was eluted in the fractions corresponding to m.w. of more than 60,000 and pI values of 5 to 6. BGDF induced both proliferation and IgM secretion in a mouse leukemic B cell line, BCL1, and these activities were not separated by either isoelectric focusing or gel filtration in the presence or absence of 0.1% Triton X-100, suggesting that the molecule designated BGDF exerted both growth and differentiation activities. BGDF acted on normal mouse B cells to induce proliferation as well as IgM secretion. The target cells of BGDF were in vivo activated B blast cells. BGDF acted on DXS-activated murine B cells to induce both proliferation and IgM secretion but not anti-Ig-activated B cells, indicating that BGDF and BSF-p1 were different molecules.