LASER FLASH PHOTOKINETIC STUDIES OF ROSE-BENGAL SENSITIZED PHOTODYNAMIC INTERACTIONS OF NUCLEOTIDES AND DNA
LASER FLASH PHOTOKINETIC STUDIES OF ROSE-BENGAL SENSITIZED PHOTODYNAMIC INTERACTIONS OF NUCLEOTIDES AND DNA
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DOI：
10.1111/j.1751-1097.1987.tb08407.x
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发表时间：
1987-01-01
影响因子：
3.3
通讯作者：
RODGERS, MAJ
中科院分区：
文献类型：
--
作者：
LEE, PCC;RODGERS, MAJ
激光闪光光解研究的生产的三重态的氧杂蒽染料，玫瑰红（RB），已进行。这种状态与氧的反应，形成单线态氧和超氧阴离子自由基已被观察到，并测量产率。RB（T1）被氧猝灭导致约75%的单线态氧和20%的超氧化物。这些物质的反应性sbd RB（T1）、O2（1 Δ g）和O2-cnt。sbd.与四个核苷酸和DNA已经确定。只有鸟嘌呤残基表现出任何显着的反应在中性pH值。在较高的pH值鸟嘌呤速率常数增加。生物光动力过程的后果进行了讨论。
Laser flash photolysis studies of the production of the triplet state of the xanthene dye, rose bengal (RB), have been carried out. The reactions of this state with oxygen to form singlet oxygen and the superoxide anion radical have been observed and yields measured. Quenching of RB(T1) by oxygen leads to approximately 75% singlet oxygen and 20% superoxide. THe reactivity of these species.sbd.RB(T1), O2(1.DELTA.g) and O2-.cntdot..sbd.with four nucleotides and DNA have been determined. Only guanine residues showed any noticeable reaction at neutral pH. At higher pH guanine rate constants increased. The consequences to biological photodynamic processes are discussed.