Salmonella enterica strains belonging to O serogroup 1,3,19 induce chlorosis and wilting of Arabidopsis thaliana leaves
Salmonella enterica strains belonging to O serogroup 1,3,19 induce chlorosis and wilting of Arabidopsis thaliana leaves
复制标题
DOI：
10.1111/j.1462-2920.2011.02429.x
复制
发表时间：
2011-05-01
影响因子：
5.1
通讯作者：
Frankel, Gad
中科院分区：
文献类型：
--
作者：
Berger, Cedric N.;Brown, Derek J.;Frankel, Gad
在过去十年里，与食用受污染的沙拉叶有关的疫情和疾病的数量急剧增加。大肠杆菌和肠沙门氏菌是与食用新鲜农产品有关的最常见的食源性病原体。肠炎链霉菌的不同血清型与沙拉叶片表面结合，表现出向气孔的趋向性，并入侵叶片并到达下面的叶肉。然而，叶片入侵的后果尚不清楚。在此，我们发现，小鼠伤寒沙门氏菌、肠炎沙门氏菌、海德堡沙门氏菌和阿戈纳沙门氏菌四个血清型，以及肠溶沙门氏菌亚种arizonae和charizonae，都能在拟南芥叶片叶肉细胞中存活下来，但不会引起叶片的黄化或枯萎。相反，S.Senftenberg在入渗后4d诱导了刺参Col-0的强叶枯萎，而在Ws-0的入渗后没有诱导出强烈的叶片枯萎。死亡的S.Senftenberg和细菌裂解物也会诱导叶片枯萎。我们发现，识别鞭毛蛋白的拟南芥病原菌相关分子模式(PAMP)识别受体(PRRS)FLS2和识别细菌延伸因子EF-Tu的EFR突变对拟南芥对S.Senftenberg的枯萎反应没有影响。与Senftenberg一样属于E-4(O：1，3，19)沙门氏菌血清组的三个血清型Cannstatt，Krefeld和利物浦对拟南欧沙门氏菌叶片的渗透也导致了叶片的迅速枯萎，而所有参试的S.Senftenberg粗株(缺乏O抗原)都不能诱导叶片枯萎。这些结果表明，沙门氏菌O抗原1，3，19特异性地触发了沙门氏菌叶片的黄化和枯萎。
P>The number of outbreaks and illness linked to the consumption of contaminated salad leaves have increased dramatically in the last decade. Escherichia coli and Salmonella enterica are the most common food-borne pathogens linked to consumption of fresh produce. Different serovars of S. enterica subspecies enterica have been shown to bind the surface of salad leaves, to exhibit tropism towards the stomata and to invade leaves and reach the underlying mesophyll. However the consequences of leaf invasion are not known. Here we show that following infiltration, serovars Typhimurium, Enteritidis, Heidelberg and Agona, as well as strains of S. enterica subspecies arizonae and diarizonae, survive in the mesophyll of Arabidopsis thaliana leaves but induce neither leaf chlorosis nor wilting. In contrast, S. Senftenberg induced strong leaf wilting 4 days post infiltration in A. thaliana accession Col-0 but not in accession Ws-0. Dead S. Senftenberg and bacterial lysates also induced leaf wilting. We found that mutations in the Arabidopsis pathogen associated molecular pattern (PAMP) recognition receptors (PRRs) FLS2, which recognizes flagellin, and EFR, which recognizes the bacterial elongation factor EF-Tu, had no effect on the wilting response of A. thaliana to S. Senftenberg. Infiltration of A. thaliana leaves with serovars Cannstatt, Krefeld and Liverpool, which like Senftenberg belong to Salmonella serogroup E-4 (O:1,3,19), also resulted in rapid leaf wilting, while all tested rough S. Senftenberg strains (lacking the O antigen) failed to elicit leaf wilting. These results suggest that the Salmonella O antigen 1,3,19 specifically triggers leaf chlorosis and wilting in A. thaliana.