Arabidopsis RAV1 is down-regulated by brassinosteroid and may act as a negative regulator during plant development
Arabidopsis RAV1 is down-regulated by brassinosteroid and may act as a negative regulator during plant development
复制标题
DOI：
10.1038/sj.cr.7290197
复制
发表时间：
2004-02-01
期刊：
影响因子：
44.1
通讯作者：
Li, JY
中科院分区：
文献类型：
--
作者：
Hu, YX;Wang, YH;Li, JY
RAV 1是一种新的DNA结合蛋白，具有两个独特的DNA结合结构域，在高等植物中是独一无二的，但其在植物生长发育中的作用尚不清楚。利用cDNA阵列技术，我们发现在拟南芥悬浮细胞中，表油菜素内酯（epiBL）下调RAV 1基因的转录。RNA凝胶印迹分析表明，epiBL调控的RAV 1转录既不涉及蛋白质磷酸化/去磷酸化，也不涉及新合成的蛋白质，也不需要功能性的BRI 1，提示这种调控可能是通过一条新的BR信号通路实现的。RAV 1在拟南芥中的过表达导致侧根和莲座叶发育的延迟，并且低表达导致早期开花表型，这意味着RAV 1可能作为生长和发育的负调控组分起作用。
RAV1 is a novel DNA-binding protein with two distinct DNA-binding domains unique in higher plants, but its role in plant growth and development remains unknown. Using cDNA array, we found that transcription of RAV1 is down-regulated by epibrassinolide (epiBL) in Arabidopsis suspension cells. RNA gel blot analysis revealed that epiBL-regulated RAV1 transcription involves neither protein phosphorylation/dephosphorylation nor newly synthesized protein, and does not require the functional BRI1, suggesting that this regulation might be through a new BR signaling pathway. Overexpressing RAV1 in Arabidopsis results in a retardation of lateral root and rosette leaf development, and the underexpression causes an earlier flowering phenotype, implying that RAV1 may function as a negative regulatory component of growth and development.