HUMAN INVIVO PHOSPHATE METABOLITE IMAGING WITH P-31 NMR
HUMAN INVIVO PHOSPHATE METABOLITE IMAGING WITH P-31 NMR
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DOI：
10.1002/mrm.1910070309
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发表时间：
1988-07-01
影响因子：
3.3
通讯作者：
MUELLER, OM
中科院分区：
文献类型：
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作者：
BOTTOMLEY, PA;CHARLES, HC;MUELLER, OM
使用脉冲相位编码梯度磁场和三维傅立叶变换 (3DFT) 技术，以 1.5 T 的功率、8.5 至 34 分钟的扫描时间、27 和 64 cm3 的空间分辨率获得了显示人脑中高能磷酸盐代谢物分布的磷 (31P) 光谱图像。数据是通过截断几何形状的正交体积 NMR 检测线圈在自由感应衰减时采集的，该线圈设计用于优化线圈轴上的信噪比，假设样本噪声代表主要噪声源，并使用自屏蔽磁场梯度线圈来最大限度地减少涡流效应。这些图像允许比较从大脑不同位置同时获取的代谢数据，以及通过在图像阵列中包含含有已知 31P 浓度标准的小瓶来量化代谢物。三个个体的 NMR 可见三磷酸腺苷值约为 3 mM 组织。 NMR可检测到的磷酸肌酸与脑中ATP的比率为1.15±。这些实验中的 SD 为 0.17。这些和其他 31P 测量中随机和系统误差的潜在来源已被识别。
Phosphorus (31P) spectroscopic images showing the distribution of high-energy phosphate metabolites in the human brain have been obtained at 1.5 T in scan times of 8.5 to 34 min at 27 and 64 cm3 spatial resolution using pulsed phase-encoding gradient magnetic fields and three-dimensional Fourier transform (3DFT) techniques. Data were acquired as free induction decays with a quadrature volume NMR detection coil of a truncated geometry designed to optimize the signal-to-noise ratio on the coil axis on the assumption that the sample noise represents the dominant noise source, and self-shielded magnetic field gradient coils to minimize eddy-current effects. The images permit comparison of metabolic data acquired simultaneously from different locations in the brain, as well as metabolite quantification by inclusion of a vial containing a standard of known 31P concentration in the image array. Values for the NMR visible adenosine triphosphate in three individuals were about 3 mM of tissue. The ratio of NMR detectable phosphocreatine to ATP in brain was 1.15 .+-. 0.17 SD in these experiments. Potential sources of random and systematic error in these and other 31P measurements are identified.