Atomic force microscopy measurement of the elastic properties of the kidney epithelial cells
Atomic force microscopy measurement of the elastic properties of the kidney epithelial cells
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DOI：
10.1016/j.jcis.2004.11.041
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发表时间：
2005-05-01
影响因子：
9.9
通讯作者：
Khan, S
中科院分区：
文献类型：
--
作者：
Rabinovich, Y;Esayanur, M;Khan, S
使用原子力显微镜（AFM）在氮化硅尖端和肾上皮细胞（Madin-Darby犬肾-MDCK和来自猪肾的近端肾小管上皮细胞，LLC-PK 1）之间进行直接相互作用力测量。考虑力/距离曲线的接近（延伸）部分，在正常条件下生长的MDCK和LLC-PK 1细胞中均观察到2-3 μ m范围内的排斥力。当细胞受损暴露底层基底膜时，还观察到50-200 nm的较短距离范围内的排斥力。LLC-PK 1细胞比MDCK细胞更容易受到损伤，因此在前者中短程力是常见的，而在后者中长程力是常见的。的功能依赖性的排斥力的压痕深度的变化，在小的压痕深度的力线性增加，而在较大的压痕的力是距离的二次函数，这是由于弹性的膜和固体样的细胞响应，分别。草酸盐处理细胞2-4小时引起细胞弹性模量的增加。(c)2004年爱思唯尔公司All rights reserved.
Direct interaction force measurements using atomic force microscopy (AFM) were carried out between a silicon nitride tip and renal epithelial cells (Madin-Darby Canine Kidney-MDCK and proximal tubular epithelial cells derived from pig kidneys, LLC-PK1). The approaching (extending) portion of the force/distance Curves is considered, and repulsive forces in the long range of 2-3 mu m were seen in both MDCK as well as LLC-PK1 cells growing under normal conditions. The repulsive force in the shorter distance range of 50-200 nm was also observed, when cells were damaged exposing the underlying basal membrane. LLC-PK1 cells were more prone to damage than the MDCK cells, hence short-range forces were common in the former and long-range forces in the latter cells. The functional dependence of repulsive force on the indentation depth changes, at small indentation depth the force increases linearly, while at larger indentations the force is a quadratic function of the distance, which is attributed to the elasticity of the membrane and the solid-like response of cells, respectively. The oxalate treatment of cells for 2-4 h gives rise to an increase in the elastic modulus of the cells. (c) 2004 Elsevier Inc. All rights reserved.