Comparison of transmission and transflectance mode FTIR imaging of biological tissue.
Comparison of transmission and transflectance mode FTIR imaging of biological tissue.
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DOI：
10.1039/c4an01975j
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发表时间：
2015-03
期刊：
影响因子：
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通讯作者：
M. Pilling;P. Bassan;P. Gardner
中科院分区：
文献类型：
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作者：
M. Pilling;P. Bassan;P. Gardner
FTIR显微镜是一种强大的技术，由于其能够在生物医学组织样品的组织病理学评估期间提供补充信息而变得流行。然而，最近，由于所谓的电场驻波（EFSW）效应，对透反射操作模式用于临床诊断的适用性提出了质疑。在本文中，我们比较了从五个不同的患者获得的前列腺组织中的透射和transflection测量的化学图像，并讨论了与每个采样方式获得的光谱的变异性。我们发现，在transflection中获得的光谱经历非线性失真，即在整个光谱的吸收带强度的非线性变化，并且有显着的差异，从同一区域的组织测量的光谱取决于操作模式。主成分分析（PCA）用于突出在透射反射模式中获得良性和癌组织之间的较差区分。此外，我们表明，使用二阶导数，而定性提高光谱歧视，并没有完全缓解根本问题。
FTIR microscopy is a powerful technique which has become popular due to its ability to provide complementary information during histopathological assessment of biomedical tissue samples. Recently however, questions have been raised on the suitability of the transflection mode of operation for clinical diagnosis due to the so called Electric Field Standing Wave (EFSW) effect. In this paper we compare chemical images measured in transmission and transflection from prostate tissue obtained from five different patients, and discuss the variability of the spectra acquired with each sampling modality. We find that spectra obtained in transflection undergo a non-linear distortion, i.e. non-linear variations in absorption band strength across the spectra, and that there are significant differences in spectra measured from the same area of tissue depending on the mode of operation. Principal Component Analysis (PCA) is used to highlight that poorer discrimination between benign and cancerous tissue is obtained in transflection mode. In addition we show that use of second derivatives, while qualitatively improves spectral discrimination, does not completely alleviate the underlying problem.