THE PHOSPHORYLASE-B TO PHOSPHORYLASE-A CONVERTING ENZYME OF RABBIT SKELETAL MUSCLE
THE PHOSPHORYLASE-B TO PHOSPHORYLASE-A CONVERTING ENZYME OF RABBIT SKELETAL MUSCLE
复制标题
DOI：
10.1016/0006-3002(56)90273-6
复制
发表时间：
1956-01-01
期刊：
影响因子：
--
通讯作者：
FISCHER, EH
中科院分区：
文献类型：
--
作者：
KREBS, EG;FISCHER, EH
描述了一种催化磷酸化酶b转化为A的酶的纯化方法。比活性提高65倍后，得到的酶不含PR酶活性。说明了几种转化酶浓度下的反应过程，并确定了转化酶的单位。酶的最适pH约为9.0；该反应需要Mn++或Mg++和三磷酸腺苷（ATP）。结果表明，单锰- atp复合物可能在反应中起作用。在P32- atp存在的情况下，磷酸化酶b转化为a，导致每摩尔磷酸化酶a至少含有2mol P32。
A method is described for the purification of the enzyme catalyzing the conversion of phosphorylase b to a. After a 65-fold increase in specific activity, the enzyme obtained is free of PR enzyme activity. The course of the reaction at several concentrations of converting enzyme is illustrated, and units of converting enzyme are defined. The optimum pH for the enzyme is approximately 9.0; the reaction requires Mn++ or Mg++ and adenosine triphosphate (ATP). It is shown that a mono-manganous-ATP complex is probably acting in the reaction. Con-version of phosphorylase b to a, carried out in the presence of P32-ATP, causes an incorporation of at least 2 moles of P32 per mole of phosphorylase a.