Quantitative determination of the pentose phosphate pathway in preimplantation mouse embryos.
Quantitative determination of the pentose phosphate pathway in preimplantation mouse embryos.
复制标题
植入前小鼠胚胎中磷酸戊糖途径的定量测定。
DOI：
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发表时间：
1986
影响因子：
3.6
通讯作者：
Raymond W. Wright
中科院分区：
文献类型：
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作者：
J. O'Fallon;Raymond W. Wright
定量计算了小鼠植入前胚胎从2-细胞期到囊胚晚期的戊糖磷酸途径（PPP）活性。这种活性随发育而变化，并表现出重复的高值和低值。在2-细胞期（15.8%）和致密桑椹胚期（13.6%）活性最高，在晚期囊胚期（3.2%）活性最低。代谢效应物二硝基苯酚（DNP）和吩嗪乙硫酸盐（PES）对PPP活性的影响相反。二硝基苯酚，虽然刺激总CO2生产，几乎消除PPP活动，而PES刺激戊糖循环活性6倍。这些结果表明PPP处于代谢控制之下，并且胚胎具有更大PPP活性的潜力。从[1- 14 C]和[6- 14 C]葡萄糖代谢获得的C-1/C-6比值与计算的PPP活性之间没有相关性。为这些实验设计了一个代谢孵育室，该孵育室具有某些独特的功能，包括连续收集14 CO2和3 H2O。将单个胚胎放置在腔室中并暂时取样用于代谢活动。随后，这些胚胎被成功地移植到假孕受体中。
A quantitative calculation was made of the pentose phosphate pathway (PPP) activity in preimplantation mouse embryos from the 2-cell through the late blastocyst stage. This activity varied with development and showed repeated high and low values. Peak activities occurred at both the 2-cell (15.8%) and compacted morula (13.6%) stages, with lowest activity at the development of the late blastocyst (3.2%). The metabolic effectors dimitrophenol (DNP) and phenazine ethosulfate (PES) had opposite effects on PPP activity. Dinitrophenol, although stimulating total CO2 production, virtually eliminated PPP activity while PES stimulated the pentose cycle activity 6-fold. These results indicated that the PPP was under metabolic control and that the embryos had a potential for much larger PPP activities. There was no correlation between the C-1/C-6 ratio obtained from the metabolism of [1-14C] and [6-14C] glucose and calculated PPP activities. A metabolic incubation chamber was devised for these experiments that exhibited certain unique features, including continuous collection of 14CO2 and 3H2O. Single embryos were placed in the chamber and sampled momentarily for metabolic activity. Subsequently, such embryos were successfully transferred to pseudopregnant recipients.
分离颗粒肺细胞中葡萄糖代谢的戊糖途径。
影响因子：
5.8
作者：
Fisher,AB;Reicherter,J
通讯作者：
Reicherter,J