The role of leptin in the development of the cerebral cortex in mouse embryos
The role of leptin in the development of the cerebral cortex in mouse embryos
复制标题
DOI：
10.1210/en.2005-0791
复制
发表时间：
2006-02-01
期刊：
影响因子：
4.8
通讯作者：
Otani, H
中科院分区：
文献类型：
--
作者：
Udagawa, J;Hashimoto, R;Otani, H
在小鼠胚胎血清中检测到瘦素，瘦素受体在小鼠胚胎大脑中表达，提示瘦素在大脑发育中起作用。与野生型相比，瘦素缺陷（ob/ob）小鼠在胚胎第16天和第18天神经上皮细胞较少，在胚胎第14天和第16天神经上皮细胞中5-溴-2 '-脱氧尿苷（+）细胞较少。在E14 ob/ob胚胎中脑室内注射瘦素增加E16时神经上皮细胞的数量。在培养的神经球细胞中，瘦素处理增加Hes 1 mRNA的表达和维持神经祖细胞。星形胶质细胞分化诱导低剂量（0.1 μ g/ml），但不是高剂量（1 μ g/ ml）瘦素。高剂量leptin降低神经球细胞Id mRNA表达，增加Ngn 1 mRNA表达。在E16和E18时，ob/ob皮质板中的神经肽Y mRNA水平低于野生型。这些结果表明，瘦素维持神经祖细胞，并与神经胶质和神经元发育的胚胎。
Leptin is detected in the sera, and leptin receptors are expressed in the cerebrum of mouse embryos, suggesting that leptin plays a role in cerebral development. Compared with the wild type, leptin-deficient (ob/ob) mice had fewer cells at embryonic day (E) 16 and E18 and had fewer 5-bromo-2'-deoxyuridine(+) cells at E14 and E16 in the neuroepithelium. Intracerebroventricular leptin injection in E14 ob/ob embryos increased the number of neuroepithelium cells at E16. In cultured neurosphere cells, leptin treatment increased Hes1 mRNA expression and maintained neural progenitors. Astrocyte differentiation was induced by low-dose (0.1 mu g/ml) but not high-dose (1 mu g/ ml) leptin. High-dose leptin decreased Id mRNA and increased Ngn1 mRNA in neurosphere cells. The neuropeptide Y mRNA level in the cortical plate was lower in ob/ob than the wild type at E16 and E18. These results suggest that leptin maintains neural progenitors and is related to glial and neuronal development in embryos.