A new in vitro assay for quantitating tumor cell invasion.
A new in vitro assay for quantitating tumor cell invasion.
复制标题
一种定量肿瘤细胞侵袭的新体外测定。
DOI：
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发表时间：
1989
期刊：
影响因子：
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通讯作者：
L. Repesh
中科院分区：
文献类型：
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作者：
L. Repesh
肿瘤细胞附着并穿透基底膜是完成转移级联反应所必需的，最终形成继发性肿瘤病灶。因此，基底膜是扩散性肿瘤细胞通过的关键屏障。我们已经开发了一个简单的，在体外模型中使用基质胶包被transwell室（Costar）用于肿瘤细胞侵袭试验。K1735紫外线诱导的小鼠黑色素瘤细胞系的两种变体进行了测定，其侵袭能力，并与其在实验性转移测定中定殖肺的能力进行了比较。K1735-M2细胞在体内具有高度转移性，在72小时测定中以显著高于低转移性细胞K1735-16的速率侵入基底膜基质胶。作为阴性对照，正常鼠成纤维细胞不能穿透屏障。体外肿瘤细胞侵袭与体内肺定植相关。因此，该模型可能为研究血行播散过程中肿瘤细胞侵袭的发病机制提供有价值的工具。
The attachment to and penetration of basement membranes by tumor cells is required to complete the metastatic cascade which culminates in the establishment of secondary tumor foci. Therefore, basement membranes are critical barriers to the passage of disseminating tumor cells. We have developed a simple, in vitro model using matrigel-coated transwell chambers (Costar) for use in a tumor cell invasion assay. Two variants of the K1735 UV-induced murine melanoma cell line were assayed for their invasive capabilities and compared with their ability to colonize the lung in an experimental metastasis assay. The K1735-M2 cells, which are highly metastatic in vivo, invaded through basement membrane matrigel at a significantly higher rate than the low metastatic cells, K1735-16, in a 72-hour assay. As a negative control, normal murine fibroblasts were incapable of penetrating the barrier. Tumor cell invasion in vitro correlated with lung colonization in vivo. Therefore, this model may provide a valuable tool to study the mechanisms involved in the pathogenesis of tumor cell invasion during hematogenous dissemination.