AN ELISA USING RECOMBINANT PROTEINS FOR THE DETECTION OF NEUTRALIZING ANTIBODIES AGAINST HUMAN CYTOMEGALOVIRUS
AN ELISA USING RECOMBINANT PROTEINS FOR THE DETECTION OF NEUTRALIZING ANTIBODIES AGAINST HUMAN CYTOMEGALOVIRUS
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DOI：
10.1002/jmv.1890390303
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发表时间：
1993-03-01
影响因子：
12.7
通讯作者：
MACH, M
中科院分区：
文献类型：
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作者：
KROPFF, B;LANDINI, MP;MACH, M
从大肠杆菌中纯化了人巨细胞病毒（HCMV）糖蛋白gp 58/116的两个融合蛋白，分别包含484-650（AD-1）和27-100（AD-2）氨基酸。大肠杆菌裂解物，并用于ELISA以测定人血清中的抗体水平。通过将116份随机选择的血清与市售HCMV-ELISA试验进行比较，确定了试验的特异性。然后将重组多肽用于分析112份人血清中的抗体滴度，并与中和HCMV的能力进行比较。观察到中和滴度与抗AD-1抗体水平之间的强相关性，而与AD-2的相关性较弱。在29份中和效价较高（>1：128）的血清中，AD-1和AD-2的阳性率分别为96%和62%，而在中和效价较低（> 1：128）的血清中，AD-1和AD-2的阳性率分别为44%和19%。
Two prokaryotically expressed fusion proteins encompassing amino acids 484-650 (AD-1) and 27-100 (AD-2) of glycoprotein gp58/116 of human cytomegalovirus (HCMV) were purified from E. coli lysates and used in ELISA to determine antibody levels in human sera. The specificity of the test was established by comparison of 116 randomly selected sera with commercially available HCMV-ELISA tests. The recombinant polypeptides were then used for the analysis of antibody titers in 112 human sera and were compared to the capacity to neutralize HCMV. A strong correlation between the neutralization titer and antibody levels against AD-1 and a weaker correlation for AD-2 was observed. Of 29 sera with a high neutralization titer (>1:128), 96% and 62% were positive for AD-1 and AD-2, respectively, while 44% and 19% were positive in sera with low neutralization titer (