CYCLIC-AMP RECEPTOR PROTEIN INTERACTS WITH LACTOSE OPERATOR DNA
CYCLIC-AMP RECEPTOR PROTEIN INTERACTS WITH LACTOSE OPERATOR DNA
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DOI：
10.1093/nar/9.2.277
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发表时间：
1981-01-01
影响因子：
14.9
通讯作者：
SCHMITZ, A
中科院分区：
文献类型：
--
作者：
SCHMITZ, A
使用“足迹”方法重新研究了大肠杆菌的野生型lac启动子-操纵子区域的环AMP受体蛋白（CRP）结合位点。发现了两个不同的相互作用位点，一个位于-60碱基对区域，这已被描述，第二个覆盖了lacoperator。这两个位点在范围、方向和CRP诱导的DNaseI增强切割位点的特征模式上相似。
The wildtypelacpromoter-operator region ofE.coliwas reinvestigated for cyclic AMP receptor protein (CRP) binding sites using the “footprinting’ method. Two distinct interaction sites were found, one in the −60 base pair region, which has previously been described, and a second one covering thelacoperator. These two sites are similar in extent, orientation and the characteristic pattern of CRP-induced DNaseI enhanced cutting sites.