Early events in the pathogenesis of avian salmonellosis
Early events in the pathogenesis of avian salmonellosis
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DOI：
10.1128/iai.67.7.3580-3586.1999
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发表时间：
1999-07-01
影响因子：
3.1
通讯作者：
Lee, MD
中科院分区：
文献类型：
--
作者：
Henderson, SC;Bouous, DI;Lee, MD
沙门氏菌是人和动物的胃肠道病原体。然而，作为宿主特异性禽类病原体的菌株在哺乳动物中通常是无毒的，而那些非特异性的菌株在家禽中是无毒的。本研究的目的是确定宿主特异性是否表现为细菌入侵上皮细胞或抵抗白细胞杀伤的能力。在这项研究中，从人和鸡中分离的白细胞用于体外杀灭沙门氏菌。鸡白痢沙门氏菌（一种禽类特异性血清型）和鼠伤寒沙门氏菌（一种广泛宿主范围的血清型）均对从两个物种中分离的多形核白细胞的杀伤敏感。两种血清型均在MQ-NCSU禽类-巨噬细胞系的细胞中复制。相反，S. pollorum对培养的上皮Henle 407、鸡肾、鸡卵巢和虎皮鹦鹉腹部肿瘤细胞是非侵入性的。然而，在鸟类挑战赛中，S。鼠伤寒沙门氏菌能迅速引起肠粘膜炎症，而S.鸡白痢在引起肠道炎症之前优先靶向法氏囊。在小鼠中引起伤寒的沙门氏菌血清型已被证明靶向肠道相关淋巴组织。本研究的观察结果表明，S。鸡白痢在雏鸡中引发的感染途径与其在肠热病病例中所采取的途径相当。
Salmonellae are gastrointestinal pathogens of man and animals. However, strains that are host-specific avian pathogens are often avirulent in mammals, and those which are nonspecific are commensal in poultry. The objective of this study was to determine whether host specificity was exhibited by bacterial abilities to invade epithelial cells or resist leukocyte killing. In this study, leukocytes isolated from humans and chickens were used to kill Salmonella in vitro. Both Salmonella pullorum, an avian-specific serotype, and Salmonella typhimurium, a broad-host-range serotype, were sensitive to killing by polymorphonuclear leukocytes isolated from both species. Both serotypes replicated in cells of the MQ-NCSU avian-macrophage cell line. In contrast, S. pollorum was noninvasive for cultured epithelial Henle 407, chick kidney, chick ovary, and budgerigar abdominal tumor cells. In the bird challenge, however, S. typhimurium rapidly caused inflammation of the intestinal mucosa, but S. pullorum preferentially targeted the bursa of Fabricius prior to eliciting intestinal inflammation. Salmonella serotypes which cause typhoid fever in mice have been shown to target the gut-associated lymphoid tissue. Observations from this study show that S. pullorum initiated a route of infection in chicks comparable to the route it takes in cases of enteric fever.