DISATURATED AND DIPOLYUNSATURATED PHOSPHOLIPIDS IN THE BOVINE RETINAL ROD OUTER SEGMENT DISK MEMBRANE
DISATURATED AND DIPOLYUNSATURATED PHOSPHOLIPIDS IN THE BOVINE RETINAL ROD OUTER SEGMENT DISK MEMBRANE
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DOI：
10.1016/0005-2736(79)90284-0
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发表时间：
1979-01-01
期刊：
影响因子：
--
通讯作者：
DRATZ, EA
中科院分区：
文献类型：
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作者：
MILJANICH, GP;SKLAR, LA;DRATZ, EA
TLC被用来分离的主要磷脂头基类的杆外段盘膜成亚馏分脂肪酸组成显着不同。至少18%的视杆外节磷脂酰胆碱必须含有2个饱和脂肪酸。此外，在至少43%的磷脂酰丝氨酸、24%的磷脂酰胆碱和24%的磷脂酰乙醇胺中发现2种不饱和脂肪酸。在所有情况下，不饱和酸主要是多不饱和的。用柠檬酸柱层析法可得到类似的分离，但分辨率较低。反式-parinaric酸（9，11，13，15-all-trans-octadecatetraenoic acid）荧光偏振的温度依赖性表明，磷脂酰胆碱亚组分水分散体的热行为与其脂肪酸组成一致。
TLC was used to separate the major phospholipid headgroup classes of the rod outer segment disc membrane into subfractions which differ markedly in fatty acid composition. At least 18% of the rod outer segment phosphatidylcholine must contain 2 saturated fatty acids. Furthermore, 2 unsaturated fatty acids are found in at least 43% of the phosphatidylserine, 24% of the phosphatidylcholine and 24% of the phosphatidylethanolamine. The unsaturated acids are predominantly polyunsaturated in all cases. A similar separation, but with less resolution, was achieved with silicic acid column chromatography. The temperature dependence of the polarization of fluorescence of trans-parinaric acid (9,11,13,15-all-trans-octadecatetraenoic acid) showed that the thermal behavior of aqueous dispersions of the phosphatidylcholine subfractions was consistent with their fatty acid compositions.