Immunoelectron microscopic studies on the specific adhesion of Trypanosoma congolense to cultured vascular endothelial cells.
Immunoelectron microscopic studies on the specific adhesion of Trypanosoma congolense to cultured vascular endothelial cells.
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刚果锥虫与培养血管内皮细胞特异性粘附的免疫电镜研究。
DOI：
10.1006/jsbi.1994.1014
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发表时间：
1994
影响因子：
3
通讯作者：
H. Risse
中科院分区：
文献类型：
--
作者：
M. Shakibaei;M. Milaninezhad;H. Risse
将血流形态的刚果锥虫与血管内皮细胞进行体外共培养。扫描和透射电子显微镜显示，锥虫特异性地附着在其鞭毛前部的内皮表面。寄生虫与寄主细胞之间的相互作用非常紧密，经常在接触部位附近观察到内吞小泡的堆积。免疫电子显微镜显示，一种化合物分布在锥虫的整个表面，并与抗β1整合素链的抗体发生反应，而与抗α1或抗α2抗体未见反应。整合素是一种典型的黏附分子，现在通过电子显微镜和免疫荧光方法证明整合素存在于弓形虫的表面。然而，这种物质是否直接参与了与内皮细胞的特异性黏附，尚不能得到证实。
Bloodstream forms of Trypanosoma congolense were cocultivated in vitro with vascular endothelial cells. The trypanosomes adhere specifically to the endothelial surfaces of the anterior part of their flagella, as shown by scanning and transmission electron microscopy. The interaction between parasite and host cell is very tight, and frequently the accumulation of endocytotic vesicles near the contact site is observed. Immunoelectron microscopy revealed a compound distributed over the total surface of the trypanosomes and reacting with antibodies against the beta 1 integrin chain, but no reaction was found with anti-alpha 1 or anti-alpha 2 antibodies. Integrins are typical adhesion molecules and are now shown to be present at the surface of T. congolense by electron microscopy and by immunofluorescence. A direct participation of this substance in the specific adhesion to endothelium, however, could not be proven.