PD-1, PD-L1 and PD-L2 expression in mouse prostate cancer.
PD-1, PD-L1 and PD-L2 expression in mouse prostate cancer.
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发表时间：
2016-01
影响因子：
1.2
通讯作者：
Shijie Yang;Qiuyang Zhang;Sen Liu;Alun R Wang;Z. You
中科院分区：
文献类型：
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作者：
Shijie Yang;Qiuyang Zhang;Sen Liu;Alun R Wang;Z. You
程序性细胞死亡蛋白 1 (PD-1) 及其配体 PD-L1 和 PD-L2 在维持免疫抑制肿瘤微环境中发挥着关键作用。本研究的目的是评估小鼠前列腺肿瘤中 PD-1、PD-L1 和 PD-L2 的表达。使用免疫组织化学染色检查了来自 Pten-null 小鼠的总共 33 个小鼠前列腺肿瘤的 PD-1、PD-L1 和 PD-L2。这些动物要么具有白细胞介素-17受体c（Il-17rc）野生型或敲除基因型，要么以常规饮食或高脂肪饮食喂养至30周龄。我们发现，Il-17rc 野生型小鼠前列腺肿瘤的 PD-1、PD-L1 和 PD-L2 水平显着高于 Il-17rc 敲除小鼠前列腺肿瘤。高脂肪饮食诱导的肥胖小鼠前列腺肿瘤中的 PD-1、PD-L1 和 PD-L2 水平显着高于常规饮食喂养的瘦小鼠。与 Il-17rc 敲除小鼠和瘦小鼠相比，PD-1、PD-L1 和 PD-L2 表达的增加分别与 Il-17rc 野生型和肥胖小鼠中形成的侵袭性前列腺肿瘤数量增加相关。我们的研究结果表明，PD-1、PD-L1 和 PD-L2 的表达可能通过创建免疫抑制肿瘤微环境来促进小鼠前列腺癌的发展。
Programmed cell death protein 1 (PD-1) and its ligands PD-L1 and PD-L2 play critical roles in maintaining an immunosuppressive tumor microenvironment. The purpose of the present study was to assess expression of PD-1, PD-L1, and PD-L2 in mouse prostate tumors. A total of 33 mouse prostate tumors derived from Pten-null mice were examined using immunohistochemical staining for PD-1, PD-L1, and PD-L2. The animals were either with interleukin-17 receptor c (Il-17rc) wild-type or knockout genotype, or fed with regular diet or high-fat diet to 30 weeks of age. We found that Il-17rc wild-type mouse prostate tumors had significantly higher levels of PD-1, PD-L1, and PD-L2 than Il-17rc knockout mouse prostate tumors. High-fat diet-induced obese mice had significantly higher levels of PD-1, PD-L1, and PD-L2 in their prostate tumors than lean mice fed with regular diet. Increased expression of PD-1, PD-L1, and PD-L2 was associated with increased number of invasive prostate tumors formed in the Il-17rc wild-type and obese mice compared to the Il-17rc knockout and lean mice, respectively. Our findings suggest that expression of PD-1, PD-L1, and PD-L2 may enhance development of mouse prostate cancer through creating an immunosuppressive tumor microenvironment.