ASURA (PHB2) Interacts with Scc1 through Chromatin
ASURA (PHB2) Interacts with Scc1 through Chromatin
复制标题
DOI：
10.1159/000350004
复制
发表时间：
2013-01-01
影响因子：
1.7
通讯作者：
Fukui, K.
中科院分区：
文献类型：
--
作者：
Equilibrina, I.;Matsunaga, S.;Fukui, K.
由内聚蛋白复合物介导的姐妹染色单体内聚对忠实染色体分离至关重要。先前我们报道了PHB2 (prohibitin2/ASURA)，一种多功能蛋白，在姐妹染色单体内聚中起作用。然而，ASURA如何参与姐妹染色单体内聚仍然不清楚。目前的共免疫沉淀分析显示ASURA在体内与黏结蛋白亚基Scc1相互作用。我们发现ASURA在整个细胞周期中以与Scc1相似的方式与染色质结合。此外，我们使用基于荧光泛素化的细胞周期指标（Fucci）系统观察发现，ASURA在有丝分裂早期的粘聚蛋白维持中很重要。我们还发现保守的PHB结构域负责ASURA的染色质靶向。我们的研究结果表明，姐妹染色单体内聚的调节是由ASURA与染色质结合介导的，其中ASURA可能通过ASURA- scc1相互作用参与内聚保护。巴塞尔S. Karger股份有限公司版权所有
Sister chromatid cohesion mediated by the cohesin complex is essential for faithful chromosome segregation. Previously we reported that PHB2 (prohibitin2/ASURA), a multifunctional protein, has a role in sister chromatid cohesion. Nevertheless, how ASURA is involved in sister chromatid cohesion still remains unclear. The present co-immunoprecipitation analysis reveals that ASURA interacts with cohesin subunit Scc1 in vivo. We show that ASURA associates with chromatin in a similar manner as Scc1 throughout the cell cycle. Furthermore, our observation using the Fucci (fluorescent ubiquitination-based cell cycle indicator) system indicates that ASURA is important for cohesin maintenance at early mitosis. We have also identified that the conserved PHB domain is responsible for chromatin targeting of ASURA. Our results suggest that the regulation of sister chromatid cohesion is mediated by ASURA binding to chromatin, where ASURA might be involved in cohesin protection through ASURA-Scc1 interactions. Copyright (C) 2013 S. Karger AG, Basel