Differentiation of human acute myeloid leukaemia cells in primary culture in response to cotylenin A, a plant growth regulator
Differentiation of human acute myeloid leukaemia cells in primary culture in response to cotylenin A, a plant growth regulator
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原代培养物中人急性髓系白血病细胞对植物生长调节剂子叶素 A 的分化
DOI：
10.1046/j.1365-2141.2001.03029.x
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发表时间：
2001
影响因子：
6.5
通讯作者：
S. Tomoyasu
中科院分区：
文献类型：
--
作者：
Kazunari Yamada;Y. Honma;K. Asahi;Takashi Sassa;K. Hino;S. Tomoyasu
Cotylenin A具有二萜类三碳环骨架，是一种植物生长调节剂，已被证明影响高等植物的几个生理过程，并在几个髓系白血病细胞系中具有诱导分化的活性。我们检测了胚胎素A对原代培养的急性髓系白血病(AML)患者新鲜分离的白血病细胞分化的影响。在12例白血病细胞中，有9例能明显促进白血病细胞的功能分化和形态分化。这种诱导分化活性比全反式维甲酸和1-α，25-二羟基维生素D3(VD3)更强。在诱导AML细胞向单核细胞分化方面，子叶素A和VD3联合作用比单独使用子叶素A或VD3更有效。
Cotylenin A, which has a diterpenoid tricarbocyclic skeleton, has been isolated as a plant growth regulator, has been shown to affect several physiological processes of higher plants and have differentiation‐inducing activity in several myeloid leukaemia cell lines. We examined the effect of cotylenin A on the differentiation of leukaemic cells that were freshly isolated from acute myeloid leukaemia (AML) patients in primary culture. Cotylenin A significantly stimulated both functional and morphological differentiation of leukaemia cells in 9 out of 12 cases. This differentiation‐inducing activity was more potent than those of all‐trans retinoic acid and 1α,25‐dihydroxyvitamin D3 (VD3). Treatment with a combination of cotylenin A and VD3 was more effective than cotylenin A or VD3 alone at inducing the monocytic differentiation of AML cells.