Dual-view oblique plane microscopy (dOPM).
Dual-view oblique plane microscopy (dOPM).
复制标题
双视图倾斜平面显微镜（DOPM）。
DOI：
10.1364/boe.409781
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发表时间：
2020-12-01
影响因子：
3.4
通讯作者：
Dunsby C
中科院分区：
文献类型：
--
作者：
Sparks H;Dent L;Bakal C;Behrens A;Salbreux G;Dunsby C
我们提出了一种新的折叠双视角斜平面显微镜(OPM)技术，称为dOPM，它可以通过在重新聚焦空间中平移一对倾斜的反射镜来获得样品的两个正交视图。以水浸40×1.15nA为主要物镜，测得2 0 0 nm微珠的半高宽为0.35 ± 0.0 4µm，横向为0.39 ± 0.0 2µm，轴向为0.81 ± 0.0 7µm。在4.99 ± 0.58µm和4.89 ± 0.63µm两个视图框中，使用光片半高宽测量的z截面值为1.33 ± 0.45µm。为了定性地证明该系统可以减少阴影伪影，同时提供更各向同性的分辨率，我们拍摄了直径约100µm的多细胞球体的图像。
We present a new folded dual-view oblique plane microscopy (OPM) technique termed dOPM that enables two orthogonal views of the sample to be obtained by translating a pair of tilted mirrors in refocussing space. Using a water immersion 40× 1.15 NA primary objective, deconvolved image volumes of 200 nm beads were measured to have full width at half maxima (FWHM) of 0.35 ± 0.04 µm and 0.39 ± 0.02 µm laterally and 0.81 ± 0.07 µm axially. The measured z-sectioning value was 1.33 ± 0.45 µm using light-sheet FWHM in the frames of the two views of 4.99 ± 0.58 µm and 4.89 ± 0.63 µm. To qualitatively demonstrate that the system can reduce shadow artefacts while providing a more isotropic resolution, a multi-cellular spheroid approximately 100 µm in diameter was imaged.