EVIDENCE FOR THE PRESENCE OF S-100 PROTEIN IN THE GLIAL COMPONENT OF THE HUMAN ENTERIC NERVOUS-SYSTEM
EVIDENCE FOR THE PRESENCE OF S-100 PROTEIN IN THE GLIAL COMPONENT OF THE HUMAN ENTERIC NERVOUS-SYSTEM
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DOI：
10.1038/297409a0
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发表时间：
1982-01-01
期刊：
影响因子：
64.8
通讯作者：
POLAK, JM
中科院分区：
文献类型：
--
作者：
FERRI, GL;PROBERT, L;POLAK, JM
近年来，肠神经系统的复杂组织结构及其在消化功能综合调控中的独立作用已被充分认识。然而，很少有人知道的非神经元的元素存在密切的形态和，据推测，与肠神经元的功能关系。虽然肠神经节中的“肠神经胶质细胞”和伴随整个胃肠壁的神经纤维的施万细胞可以根据其超微结构特征通过电子显微镜容易地识别1 -3，但是缺乏用于肠神经系统的这些非神经元组分的可视化的合适的共同标记物阻碍了研究。S-100是一种最初从脑中分离的Ca 2+结合蛋白4 5，主要位于神经胶质中6，7。我们在这里证明，使用免疫组织化学和电子免疫细胞化学，肠神经胶质细胞和雪旺细胞的人肠道含有密集的免疫反应性S-100。因此，这种蛋白质可以被认为是肠神经系统的神经胶质成分的共同标志物。
Recently, the complex organization of the enteric nervous system and its independent role in the integrative control of digestive functions have been fully recognized. However, little is known about the non-neuronal elements present in close morphological and, presumably, functional relationship with the enteric neurones. Although ‘enteric glial cells’ in gut ganglia and Schwann cells accompanying nerve fibres throughout the gastrointestinal wall can easily be recognized by electron microscopy on the basis of their ultrastructural features1–3, the lack of a suitable common marker for the visualization of these non-neuronal components of the enteric nervous system has hampered investigation. S-100 is a Ca2+-binding protein4originally isolated from the brain5, where it is localized mainly in glia6,7. We demonstrate here, using immunohistochemistry and electron immunocytochemistry, that both enteric glial cells and Schwann cells of the human gut contain densely immunoreactive S-100. This protein can therefore be regarded as a common marker for the glial components of the enteric nervous system.