SPECIFIC CHEMOTHERAPY OF CHAGAS-DISEASE - A COMPARISON BETWEEN THE RESPONSE IN PATIENTS AND EXPERIMENTAL-ANIMALS INOCULATED WITH THE SAME STRAINS
SPECIFIC CHEMOTHERAPY OF CHAGAS-DISEASE - A COMPARISON BETWEEN THE RESPONSE IN PATIENTS AND EXPERIMENTAL-ANIMALS INOCULATED WITH THE SAME STRAINS
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DOI：
10.1016/0035-9203(92)90156-7
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发表时间：
1992-11-01
影响因子：
2.2
通讯作者：
LUQUETTI, AO
中科院分区：
文献类型：
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作者：
ANDRADE, SG;RASSI, A;LUQUETTI, AO
通过异种诊断和接种新生小鼠，从巴西中部查加斯病患者中分离出11株克氏锥虫。生物学特性和同工酶分析结果表明，6株为Ⅱ型（酶模式2），5株为Ⅲ型（酶模式1）。患者接受苯硝咪唑或苯硝咪唑加硝呋替莫治疗。处理感染每种分离菌株的小鼠，以与在相应患者中获得的结果进行比较。根据间接免疫荧光试验、补体结合反应和异种诊断对患者的治愈情况进行评估。对于小鼠，使用了血培养、间接免疫荧光试验、异种诊断和新生小鼠血液接种。在治疗结束后3 - 6个月进行测试。感染II型菌株的小鼠治愈率为66 - 100%，感染III型菌株的小鼠治愈率为0 - 9%。患者和小鼠的治疗结果之间的相关性为81.8%（11例中的9例）。II型菌株对治疗更敏感，与产生大多数治疗失败的III型菌株相反。
Eleven strains of Trypanosoma cruzi were isolated from patients with Chagas disease in central Brazil by xenodiagnosis and inoculation into newborn mice. Biological characterization and isoenzyme analysis showed that 6 strains were type II (zymodeme 2) and 5 were type III (zymodeme 1). Patients were treated with benznidazole or benznidazole plus nifurtimox. Mice infected with each isolated strain were treated for comparison with the results obtained in the respective patient. Evaluation of cure of the patients was based on the indirect immunofluorescence test, complement fixation reaction and xenodiagnosis. For the mice, haemoculture, indirect immunofluorescence testing, xenodiagnosis and inoculation of blood into newborn mice were used. Tests were performed 3-6 months after the end of treatment. The cure rate was 66-100% in mice infected with type II strains and 0-9% in those infected with type III strains. The correlation between treatment results in patients and mice was 81.8% (9 of 11 cases). Type II strains were more susceptible to treatment, in contrast to type III strains which yielded the majority of therapeutic failures.