Trace element mapping of a single cell using a hard x-ray nanobeam focused by a Kirkpatrick-Baez mirror system
Trace element mapping of a single cell using a hard x-ray nanobeam focused by a Kirkpatrick-Baez mirror system
复制标题
DOI：
10.1002/xrs.1123
复制
发表时间：
2009-03-01
影响因子：
1.2
通讯作者：
Yamauchi, K.
中科院分区：
文献类型：
--
作者：
Matsuyama, S.;Shimura, M.;Yamauchi, K.
To visualize the distributions of trace elements in biological samples such as tissues and cells at high spatial resolution, we developed a scanning x-ray fluorescence microscope (SXFM) at SPring-8, using a Kirkpatrick-Baez mirror optics that enables achromatic and highly efficient focusing. To evaluate performance regarding its application to biological samples, the SXFM was used at x-ray energy of 15 keV to observe NIH/3T3 cells in which adenosine triphosphate (ATP) synthase beta (specifically localized at the mitochondria) were labeled with gold colloidal particles. Various elemental distributions were visualized at the single-cell level, including those for P, S, Cl, Ca, Fe, Cu, Zn and Au, and we obtained high-resolution elemental distribution maps by magnifying the labeled single mitochondrion. Maximum spatial resolution achieved in the experiments was sub-100 nm. Copyright (C) 2008 John Wiley & Sons, Ltd.
