The appearance of hypodense eosinophils in antigen-dependent late phase asthma.
The appearance of hypodense eosinophils in antigen-dependent late phase asthma.
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抗原依赖性晚期哮喘中低密度嗜酸性粒细胞的出现。
DOI：
10.1164/ajrccm/139.6.1401
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Busse,WW
中科院分区：
文献类型：
--
作者：
Frick,WE;Sedgwick,JB;Busse,WW
尽管哮喘患者外周血低密度嗜酸性粒细胞（EOS）增加，但其机制尚未完全确定。本研究评价了抗原支气管激发（BP）对哮喘患者循环低密度嗜酸性粒细胞（HE）百分比的影响。在抗原BP前即刻和抗原BP后24小时，通过在多重不连续密度Pereoll梯度上离心外周血粒细胞来测量EOS密度。我们发现，当在抗原BP后24小时进行评估时，外周血HE（密度< 1.095 g/ml）的百分比比基线值（78.9±4.8%对53.3± 8.2%，平均值±SEM，n= 11）显著增加（p< 0.02），但仅在同时经历即刻（IAR）和晚期哮喘反应（LAR）的患者中。在仅对吸入抗原有早期哮喘反应的哮喘受试者中，未观察到HE百分比的显著变化（n= 6）。在一个扩大的过敏性哮喘人群中（n= 38），发现外周血HE百分比与疾病严重程度（以FEV百分比预测值表示）之间存在显著相关性（r=-0.56，P = 0.003）。这些数据表明，吸入抗原在体内激活哮喘可增加低密度外周血EOS的比例，但仅限于IAR和LAR患者。此外，我们还得出结论，HE的百分比更好地反映了哮喘的严重程度比总外周血嗜酸性粒细胞的浓度。由于HE在代谢上可能比其正常致密对应物更活跃和更促炎，我们推测HE可能是导致LARs和可能的哮喘严重程度的级联事件的重要参与者。AM修订版DIS 1989; 139：1401-1406
Although peripheral blood eosinophils (EOS) of low density are increased in patients with asthma, the mechanisms contributing to their presence are not well established. The following study evaluated the effect of an antigen bronchoprovocation (BP) on the percentage of cirCulating hypodense eosinohpils (HE) in asthma. EOS density was measured by centrifugation of peripheral blood granulocytes over mUltiple discontinuous density Pereoll gradients in samples taken Immediately before and 24 h after antigen BP. We found that the percentage of peripheral blood HE (density< 1.095 g/ml) increased significantly (p< 0.02) over baseline values (78.9±4.8% versus 53.3±8.2%, mean±SEM, n= 11) when evaluated 24 h after antigen BP but only in patients who experienced both an immediate (IAR) and late phase asthma reaction (LAR). No significant change In the percentage of HE was observed in asthma subjects who had only an early asthma response to Inhaled antigen (n= 6). In an expanded population of allergic asthmatics (n= 38), a significant correlation was found between the percent peripheral blood HE and disease severity as represented by the percent predicted FEV,(r=-0.56, P= 0.003). These data suggest that in riro activation of asthma by inhaled antigen Increases the proportion of peripheral blood EOS that are hypodense but only In those patients with both an IAR and LAR. Furthermore, we also conclude that the percentage of HE better reflects the severity of asthma than the concentration of total peripheral blood eosinophils. Because the HE may be metabolically more active and prolnflammatory than its normal dense counterpart, we have speculated that the HE may be an important participant in the cascade of events causing LARs and possibly asthma severity. AM REV RESPIR DIS 1989; 139: 1401-1406