Grape seed proanthocyanidin-loaded gel-like W/O/W emulsion stabilized by genipin-crosslinked alkaline soluble polysaccharides-whey protein isolate conjugates: Fabrication, stability, and in vitro digestion
Grape seed proanthocyanidin-loaded gel-like W/O/W emulsion stabilized by genipin-crosslinked alkaline soluble polysaccharides-whey protein isolate conjugates: Fabrication, stability, and in vitro digestion
复制标题
由京尼平交联碱性可溶性多糖-乳清分离蛋白缀合物稳定的葡萄籽原花青素凝胶状 W/O/W 乳液：制备、稳定性和体外消化
DOI：
10.1016/j.ijbiomac.2021.07.062
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发表时间：
2021-07-17
影响因子：
8.2
通讯作者：
Yu, Shujuan
中科院分区：
文献类型：
--
作者：
Huang, Yaocheng;Lin, Jiawei;Yu, Shujuan
本研究旨在制备膝平素交联碱性可溶性多糖乳清分离蛋白偶联物（G-AWC），以稳定葡萄籽原花青素（GSP）包封和递送的W/O/W乳状液。交联反应后，分子量增加，表面疏水性降低。然后，采用G-AWC和聚糖聚蓖麻油酸酯（PGPR，一种亲脂乳化剂），在W-1相中加入明胶和蔗糖，通过两步法制备gsp负载W/O/W乳液。当W-1/O体积分数（Phi）为10% ~ 70%时，可制备出乳化状乳液，再将其提高到75%甚至90%，可得到具有明显弹性行为的凝胶状乳液。在φ为80%的W-1/O/W-2乳液中，GSP的包封率（EE）可达95.86%，贮存一周后EE下降约10%。此外，包封后的GSP在模拟胃肠道消化中的生物可达性（40.72%）显著高于游离GSP（13.11%）。结果表明，g - awc稳定的水/水/水乳剂是一种有效的水溶性生物活性化合物包封载体，具有较好的稳定性和生物可及性。
The present work aims to fabricate the genipin-crosslinked alkaline soluble polysaccharides-whey protein isolate conjugates (G-AWC) to stabilize W/O/W emulsions for encapsulation and delivery of grape seed proanthocyanidins (GSP). After crosslinking reaction, the molecular weight was increased and surface hydrophobicity was decreased. Then, the G-AWC and polyglyceml polyricinoleate (PGPR, a lipophilic emulsifier) were employed to prepare a GSP-loaded W/O/W emulsion with the addition of gelatin and sucrose in W-1 phase via a two-step procedure. Creamed emulsion could be fabricated at W-1/O volume fraction (Phi) of 10%-70% and further increased (Phi) to 75% or even up to 90% could obtain gel-like emulsion with notably elastic behaviors. In the W-1/O/W-2 emulsion with (Phi) of 80%, the encapsulation efficiency (EE) of GSP reached up to 95.86%, and decreased by ca. 10% after a week of storage. Moreover, the encapsulated GSP in the emulsion showed a remarkably higher bioaccessibility (40.72%) compared to free GSP (13.11%) in the simulated gastrointestinal digestion. These results indicated that G-AWC-stabilized W/O/W emulsions could be an effective carrier to encapsulate water-soluble bioactive compounds with enhanced stability and bioaccessibility.