An siRNA ribonucleoprotein is found associated with polyribosomes in Trypanosoma brucei
An siRNA ribonucleoprotein is found associated with polyribosomes in Trypanosoma brucei
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作者：
Djikeng, A;Shi, HF;Ullu, E
通过双链RNA（dsRNA）或RNA干扰（RNAi）的遗传干扰触发细胞转录物的序列特异性降解。mRNA降解的介质是小干扰RNA（siRNA）。在这里，我们报告说，在布氏锥虫，10%-20%的siRNA与多聚核糖体共沉积。阻止核糖体在mRNA上的组装会导致SiRNA沉积为高分子量复合物的同时减少。我们进一步提供的证据表明，siRNA与翻译核糖体，这种协会是由70 kD的核糖核蛋白复合物介导的。
Genetic interference by double-stranded RNA (dsRNA) or RNA interference (RNAi) triggers the sequence-specific degradation of cellular transcripts. The mediators of mRNA degradation are small interfering RNAs (siRNAs). Here, we report that in Trypanosoma brucei, 10%-20% of siRNAs cosediment with polyribosomes. Preventing the assembly of ribosomes on mRNAs results in a concomitant decrease of siRNAs sedimenting as high-molecular-weight complexes. We further provide evidence that siRNAs are associated with translating ribosomes and that this association is mediated by a 70-kD ribonucleoprotein complex.