ABC Transporters Involved in the Biogenesis of the Outer Membrane in Gram-Negative Bacteria
ABC Transporters Involved in the Biogenesis of the Outer Membrane in Gram-Negative Bacteria
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DOI：
10.1271/bbb.110115
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发表时间：
2011-06
期刊：
影响因子：
--
通讯作者：
Shin-ichiro Narita
中科院分区：
文献类型：
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作者：
Shin-ichiro Narita
革兰氏阴性菌的外膜是含有磷脂和脂多糖（LPS）的不对称脂质双层。 β-桶状外膜蛋白和脂蛋白嵌入外膜中。所有这些成分对于外膜的功能都是必不可少的。脂蛋白的转运系统已被详细表征。 ATP 结合盒 (ABC) 转运蛋白 LolCDE 通过介导脂蛋白与内膜分离以在周质中形成水溶性脂蛋白-LolA 复合物来启动分选。然后脂蛋白被转移到外膜的 LolB 并掺入脂质双层中。有人提出了一种类似于 Lol 系统的模型用于 LPS 的转运，其中 ABC 转运蛋白 LptBFG 介导 LPS 从内膜脱离。讨论了参与革兰氏阴性菌外膜生物发生的 ABC 转运蛋白功能表征的最新进展。
The outer membrane of gram-negative bacteria is an asymmetric lipid bilayer with phospholipids and lipopolysaccharides (LPSs). β-Barreled outer membrane proteins and lipoproteins are embedded in the outer membrane. All of these constituents are essential to the function of the outer membrane. The transport systems for lipoproteins have been characterized in detail. An ATP-binding cassette (ABC) transporter, LolCDE, initiates sorting by mediating the detachment of lipoproteins from the inner membrane to form a water-soluble lipoprotein-LolA complex in the periplasm. Lipoproteins are then transferred to LolB at the outer membrane and are incorporated into the lipid bilayer. A model analogous to the Lol system has been suggested for the transport of LPS, where an ABC transporter, LptBFG, mediates the detachment of LPS from the inner membrane. Recent developments in the functional characterization of ABC transporters involved in the biogenesis of the outer membrane in gram-negative bacteria are discussed.