CHARACTERISTIC BIOCHEMICAL DIFFERENCES IN HUMAN LYMPHOCYTES-T AND LYMPHOCYTES-B SEPARATED ON NYLON WOOL
CHARACTERISTIC BIOCHEMICAL DIFFERENCES IN HUMAN LYMPHOCYTES-T AND LYMPHOCYTES-B SEPARATED ON NYLON WOOL
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DOI：
10.1016/0020-711x(85)90094-1
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发表时间：
1985-01-01
期刊：
影响因子：
--
通讯作者：
ANTONI, F
中科院分区：
文献类型：
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作者：
HRABAK, A;SZABO, MT;ANTONI, F
用尼龙棉柱分离人扁桃体T、B淋巴细胞。T细胞在非粘附部分中富集，在粘附部分中富集B细胞。在分离和未分离的淋巴细胞群中检测了几种酶和其他标志物。某些酶和其他性质可以用于T或B淋巴细胞标记，因为它们优先出现，并且因为它们的估计优点（简单，快速的方法，客观的评估）。根据结果，将以下特征视为标志物：T淋巴细胞中的酸性磷酸酶、Na+-K+激活ATP酶、BAEE[N-苯甲酰基-乙酰基-乙基酯]-肽酶和铬标记; B淋巴细胞中的5“-核苷酸酶、FITC[异硫氰酸荧光素]-IgG结合、N-乙酰基-D-氨基葡萄糖苷酶、胸苷和缬氨酸掺入。
T and B lymphocytes from human tonsils were separated on a nylon wool column. T cells are enriched in the nonadherent, B cells in the adherent fraction. Several enzymes and other markers were tested in separated and nonseparated lymphocyte populations. Certain enzymes and other properties can be used at T or B lymphocyte markers because of their preferential occurrence and because of the advantages of their estimation (simple, quick methods, objective evaluation). The following characteristics were considered as markers based on the results: acid phosphatase, Na+-K+-activated ATPase, BAEE[N-benzoyl-arginyl-ethylester]-peptidase and chromium labeling in T lymphocytes; 5''-nucleotidase, FITC[fluorescein isothiocyanate]-IgG binding, N-acetyl-D-glucosaminidase, thymidine and valine incorporation in B lymphocytes.