Identification of Genes Downstream of Nodal in the Ciona intestinalis Embryo
Identification of Genes Downstream of Nodal in the Ciona intestinalis Embryo
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DOI：
10.2108/zsj.27.69
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发表时间：
2010-02
影响因子：
3.4
通讯作者：
Kaoru Mita;R. Koyanagi;K. Azumi;S. Sabau;S. Fujiwara
中科院分区：
文献类型：
--
作者：
Kaoru Mita;R. Koyanagi;K. Azumi;S. Sabau;S. Fujiwara
结节是一种生长因子，属于转化生长因子超家族，是肠轮神经管形成所必需的。先前的研究已经揭示了许多基因在Ciona胚胎中的表达受Nodal控制；然而，所有这些基因都编码转录因子和信号分子。在本研究中，我们发现了5个受Nodal基因过表达影响的基因，这些基因在肠管弯曲菌的胚胎中过表达。上调的基因包括编码IV型胶原1/3/5、层粘连蛋白-5和皮刺的基因。下调的基因包括编码Glypcan和1-Protocadherln样蛋白的基因。其中许多基因在原肠胚晚期的神经板中表达。本研究揭示了直接调节肠乔纳神经管形态发生的结节反应的候选效应基因。
Nodal, a growth factor belonging to the TGF- superfamily, is required for the formation of the neural tube in Ciona intestinalis. Previous studies have revealed many genes whose expression is controlled by Nodal in the Ciona embryo; however, all of them encode transcription factors and signaling molecules. In the present study, we identified five genes upregulated or downregulated by the overexpression of Nodal in embryos of C. intestinalis. The upregulated genes included those encoding type IV collagen 1/3/5, laminin-5, and Prickle. The downregulated genes included those encoding glypican and 1-protocadherln-like. Many of these genes were expressed in the neural plate at the late gastrula stage. The present study revealed candidate effector genes that directly regulate, in response to Nodal, the morphogenesis of the neural tube in Ciona intestinalis.