A Fluorescent Sensor and Gel Stain for Detection of Pyrophosphorylated Proteins.
A Fluorescent Sensor and Gel Stain for Detection of Pyrophosphorylated Proteins.
复制标题
用于检测焦磷酸化蛋白质的荧光传感器和凝胶染色剂。
DOI：
10.1021/acschembio.5b00256
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发表时间：
2015
影响因子：
4
通讯作者：
Fiedler,Dorothea
中科院分区：
文献类型：
--
作者：
Williams,FlorenceJ;Fiedler,Dorothea
The design and synthesis of a fluorescent sensor of diphosphate esters along with its application for in-gel detection is described. Dinuclear zinc complex1selectively binds diphosphate esters in the presence of various other functional groups, including monophosphate esters. Complex1also constitutes a competent stain for visualization of pyrophosphorylated proteins in polyacrylamide gels. This reagent will facilitate the validation and exploration of protein pyrophosphorylation.
