A novel role for aquaporin-5 in enhancing microtubule organization and stability.
A novel role for aquaporin-5 in enhancing microtubule organization and stability.
复制标题
DOI：
10.1371/journal.pone.0038717
复制
发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
King LS
中科院分区：
文献类型：
--
作者：
Sidhaye VK;Chau E;Srivastava V;Sirimalle S;Balabhadrapatruni C;Aggarwal NR;D'Alessio FR;Robinson DN;King LS
水通道蛋白5（AQP5）是位于气道上皮细胞顶端的水特异性通道。除了调节跨细胞的水渗透性，AQP5可以调节细胞旁的渗透性，虽然发生这种情况的机制尚未确定。微管还调节细胞旁渗透性。在这里，我们报告说，AQP5促进微管组装，并有助于维持组装微管稳态水平，细胞中的周转动力学较慢。具体而言，AQP5水平降低与组装微管水平降低和细胞旁通透性降低相关。相反，AQP5的过表达增加微管的组装，MT稳定性增加的证据，并促进长直微管在上皮细胞的顶端域的形成。这些发现表明，AQP5介导的微管动力学调节气道上皮屏障特性和上皮功能。
Aquaporin-5 (AQP5) is a water-specific channel located on the apical surface of airway epithelial cells. In addition to regulating transcellular water permeability, AQP5 can regulate paracellular permeability, though the mechanisms by which this occurs have not been determined. Microtubules also regulate paracellular permeability. Here, we report that AQP5 promotes microtubule assembly and helps maintain the assembled microtubule steady state levels with slower turnover dynamics in cells. Specifically, reduced levels of AQP5 correlated with lower levels of assembled microtubules and decreased paracellular permeability. In contrast, overexpression of AQP5 increased assembly of microtubules, with evidence of increased MT stability, and promoted the formation of long straight microtubules in the apical domain of the epithelial cells. These findings indicate that AQP5-mediated regulation of microtubule dynamics modulates airway epithelial barrier properties and epithelial function.
登录
查看更多内容
影响因子：
3.7
作者：
Chae YK;Woo J;Kim MJ;Kang SK;Kim MS;Lee J;Lee SK;Gong G;Kim YH;Soria JC;Jang SJ;Sidransky D;Moon C
通讯作者：
Moon C
DOI：
10.1165/rcmb.2003-0432oc
发表时间：
2004-11-01
影响因子：
6.4
作者：
Kawkitinarong, K;Linz-McGillem, L;Garcia, JGN
通讯作者：
Garcia, JGN
影响因子：
4.8
作者：
Ma, TH;Song, YL;Verkman, AS
通讯作者：
Verkman, AS
DOI：
10.1073/pnas.95.6.2991
发表时间：
1998-03-17
影响因子：
11.1
作者：
Dobbs, LG;Gonzalez, R;Verkman, AS
通讯作者：
Verkman, AS
影响因子：
3.7
作者：
Chae YK;Kang SK;Kim MS;Woo J;Lee J;Chang S;Kim DW;Kim M;Park S;Kim I;Keam B;Rhee J;Koo NH;Park G;Kim SH;Jang SE;Kweon IY;Sidransky D;Moon C
通讯作者：
Moon C