Gene Cloning and Properties of the RND‐Type Multidrug Efflux Pumps MexPQ‐OpmE and MexMN‐OprM from Pseudomonas aeruginosa
Gene Cloning and Properties of the RND‐Type Multidrug Efflux Pumps MexPQ‐OpmE and MexMN‐OprM from Pseudomonas aeruginosa
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DOI：
10.1111/j.1348-0421.2005.tb03696.x
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发表时间：
2005-11
影响因子：
2.6
通讯作者：
Takehiko Mima;H. Sekiya;T. Mizushima;T. Kuroda;T. Tsuchiya
中科院分区：
文献类型：
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作者：
Takehiko Mima;H. Sekiya;T. Mizushima;T. Kuroda;T. Tsuchiya
我们用PCR方法从铜绿假单胞菌中克隆了两个推测为RND型多药外排泵的操纵子。我们在一个操纵子mexPQ（‐opmE）和另一个操纵子mexMN中指定了这些基因。将mexPQ - opmE引入药物过敏细胞可导致大环内酯类药物、氟喹诺酮类药物和其他一些药物的mic升高。将mexMN引入含有oprM的过敏细胞，而不引入不含oprM的细胞，导致氯霉素和硫霉素的mic升高。因此，我们得出结论，MexPQ‐OpmE和MexMN‐OprM在铜绿假单胞菌中表达时是功能性的多药物外排泵。
We cloned two operons for putative RND‐type multidrug efflux pumps from Pseudomonas aeruginosa by a PCR method. We designated the genes in one operon mexPQ(‐opmE) and in another operon mexMN. Introduction of the mexPQ‐opmE into drug hypersensitive cells resulted in elevated MICs of macrolides, fluoroquinolones and some other drugs. Introduction of the mexMN into the hypersensitive cells possessing oprM, but not into cells not possessing oprM, resulted in elevated MICs of chloramphenicol and thiamphenicol. Thus, we conclude that MexPQ‐OpmE and MexMN‐OprM are functional multidrug efflux pumps when expressed in P. aeruginosa.