Removal of 2 cells from cleavage stage embryos is likely to reduce the efficacy of chromosomal tests that are used to enhance implantation rates
Removal of 2 cells from cleavage stage embryos is likely to reduce the efficacy of chromosomal tests that are used to enhance implantation rates
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DOI：
10.1016/j.fertnstert.2006.07.1516
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发表时间：
2007-03-01
影响因子：
6.7
通讯作者：
Munne, Santiago
中科院分区：
文献类型：
--
作者：
Cohen, Jacques;Wells, Dagan;Munne, Santiago
目的：评价涉及胚胎植入前遗传学诊断的染色体检测研究之间的结果差异是否受到技术的影响，如活检细胞的数量或研究设计的差异。设计：根据探针的使用、固定技术、误差测定和分析的每个胚胎的细胞数量来评估非整倍体检测的研究。环境：胚胎植入前遗传学诊断实验室。患者：在已发表的研究中，因不孕症或反复流产接受植入前遗传学诊断的患者。干预措施：根据每项评估的研究，通过胚胎冷冻和部分细胞丢失模型的比较来评估活检细胞的数量及其对进一步发育的影响。主要结局指标：探针的使用，固定策略，活检细胞的数量，不同已发表研究的错误率测定。结果(S)：研究之间的结果差异可以用所使用的技术来解释，并不一定受到设计和患者分配差异的影响。结论：与非整倍体筛查改善着床和降低流产率的发现相矛盾的研究都具有以下方面的>= 1的共同点：(1)过量的细胞被移除；（II）探针选择不当；（三）次优固定技术。[j] .中国农业科学（英文版），2007;22(1):1 - 4。(c) 2007年，美国生殖医学学会。)
Objective: To evaluate whether differences in results between studies that involve preimplantation genetic diagnosis for chromosome testing are affected by technology, such as the number of cells to be biopsied or by differences in study design.Design: Evaluation of studies of aneuploidy testing according to the use of probes, fixation technology, error determination, and number of cells per embryo analyzed.Setting: Preimplantation genetic diagnosis laboratories.Patient(s): Patients in published studies who underwent preimplantation genetic diagnosis for infertility or repeated pregnancy loss.Intervention(s): As determined by each evaluated study, the number of biopsied cells and its effect on further development was evaluated by a comparison of models of embryo freezing and partial cell loss.Main Outcome Measure(s): Use of probes, fixation strategy, number of biopsied cells, and error rate determination of different published studies.Result(S): Differences in results between studies can be explained by the technology used and are not affected necessarily by differences in design and patient allocation.Conclusion(s): Studies that contradict the finding that aneuploidy screening improves implantation and lowers miscarriage rates all have >= 1 of the following aspects in common: (I) an excess of cells having been removed; (II) inadequate choice of probes; and (III) suboptimal fixation technology. (Fertil Steril (R) 2007;87:496-503. (c) 2007 by American Society for Reproductive Medicine.)