In situ characterization of T lymphocyte subsets in the reactional states of leprosy.
In situ characterization of T lymphocyte subsets in the reactional states of leprosy.
复制标题
麻风病反应状态下 T 淋巴细胞亚群的原位表征。
DOI：
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发表时间：
1983
影响因子：
4.6
通讯作者：
T. Rea
中科院分区：
文献类型：
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作者：
R. Modlin;J. Gebhard;C. Taylor;T. Rea
应用单抗和免疫过氧化物酶技术，对6例逆转反应、9例结节性红斑麻风和2例Lucio‘s反应的麻风反应期组织中T淋巴细胞亚群的数量和分布进行了检测，并与稳定期、无反应期麻风患者(6例结节样麻风、2例交界性麻风和7例麻风)进行了比较。无论是无反应性结核样皮损还是逆转反应，肉芽肿周边的抑制子/细胞毒表型分离模式均可见，但前者更为明显。在Enl和Lucio的反应中，以及在非反应性麻风组织中，辅助/诱导型和抑制型/细胞毒表型都与聚集的组织细胞混合。而非反应性麻风组织中的辅助/抑制比(2.1+/-0.4)显著高于非反应性麻风组织(0.7+/-0.4，P<0.001)。未成熟胸腺细胞抗原OKT6分布于散在的大非淋巴样细胞，多见于类结核和逆转反应组织，较少见于ENL，但仅不规则地见于非反应性麻风组织。抑制子/细胞毒性表型的外周模式可能是细胞介导的免疫反应的免疫组织学反映，在无反应性结核样和逆转反应患者中都是如此。与非反应性麻风疾病相比，ENL中辅助/抑制比的逆转表明细胞免疫在ENL的发病机制中发挥了一定的作用。OKT6阳性细胞的来源和功能未知。
Using monoclonal antibodies and the immunoperoxidase technique, the numbers and distribution of T lymphocyte subsets in the tissues of reactional states of leprosy (six reversal reaction, nine erythema nodosum leprosum (ENL) and two Lucio's reaction) were determined and compared with those found in stable, non-reactional patients (six tuberculoid, two borderline lepromatous and seven lepromatous). The pattern of segregation of the suppressor/cytotoxic phenotype at the periphery of the granuloma was found in both non-reactional tuberculoid lesions and reversal reactions, but was better developed in the former. In ENL and Lucio's reaction, as well as in non-reactional lepromatous tissue, the helper/inducer and suppressor/cytotoxic phenotypes were both admixed with the aggregated histiocytes. However, the helper/suppressor ratio in ENL (2.1 +/- 0.4) was significantly larger than that in non-reactional lepromatous tissue (0.7 +/- 0.4, P less than 0.001). The immature thymocyte antigen OKT6 was found on scattered large non-lymphoid cells, most commonly in tuberculoid and reversal reaction tissues, less commonly in ENL, but only irregularly in non-reactional lepromatous tissue. The peripheral pattern of the suppressor/cytotoxic phenotype may be an immunohistological reflection of a cell-mediated immune response common to both non-reactional tuberculoid and reversal reaction patients. The reversal of the helper/suppressor ratio in ENL as compared to non-reactional lepromatous disease suggests some role for cell-mediated immunity in the pathogenesis of ENL. The OKT6 positive cell is of unknown origin and function.