The peptide encoded by a novel putative lncRNA HBVPTPAP inducing the apoptosis of hepatocellular carcinoma cells by modulating JAK/STAT signaling pathways
The peptide encoded by a novel putative lncRNA HBVPTPAP inducing the apoptosis of hepatocellular carcinoma cells by modulating JAK/STAT signaling pathways
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DOI：
10.1016/j.virusres.2020.198104
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发表时间：
2020-10-02
期刊：
影响因子：
5
通讯作者：
Cheng, Jun
中科院分区：
文献类型：
--
作者：
Lun, Yong-Zhi;Pan, Zhi-Peng;Cheng, Jun
当乙肝病毒进入靶细胞时，病毒与靶细胞之间的相互作用涉及复杂的反式调节机制。本研究利用抑制性消减杂交技术从肝母细胞瘤细胞系HepG2中筛选出一个新基因，称为lncRNA HBVPTPAP，用于研究HBVPTPAP对HBVDNA聚合酶的反式调节作用。根据全长序列的结构特征，将其归类为长非编码RNA。然而，一个独特而完整的开放阅读框架(ORF)仍然存在。因此，为了进一步确定lncRNA HBVPTPAP基因的编码潜力，本研究利用多种在线工具对其编码多肽的真实性进行了分析和验证。在此基础上，研究了lncRNA HBVPTPAP基因对HepG2细胞生物学行为的影响及其分子调控机制。研究发现，LncRNA HBVPTPAP亚细胞主要位于细胞质中，可能通过编码多肽与PILRA胞内结构域相互作用激活下游JAK/STAT信号通路。那么，线粒体的凋亡途径可能已经启动，从而诱导了细胞的凋亡。这些结果为进一步研究lncRNA HBVPTPAP基因的生物学功能奠定了基础。
When the hepatitis B virus (HBV) enters target cells, there are complex trans-regulatory mechanisms involved in the interactions between the virus and the target cells. In the present study, a new gene screened from the hepatoblastoma cell line HepG2 using suppression subtractive hybridization, referred to as lncRNA HBVPTPAP, was used to study the trans-regulation of HBV DNA polymerase. According to the structural characteristics of the full-length sequences, it was classified as long non-coding RNA. However, a unique and complete open reading frame (ORF) was still present. Therefore, to further identify the lncRNA HBVPTPAP gene's encoding potential, this study used several online tools to analyze and verify its encoding polypeptide authenticity. On that basis, the effects of the lncRNA HBVPTPAP gene on the biological behaviors of HepG2 cells and its molecular regulatory mechanism were investigated. It was found that the lncRNA HBVPTPAP subcellular was mainly located in the cytoplasm, and possibly activated the downstream JAK/STAT signaling pathway through the interaction between the encoding polypeptide and PILRA intracellular domain. Then, the mitochondrial apoptosis pathway may have been initiated to induce apoptosis. These results provided a basis for further study of the biological functions of the lncRNA HBVPTPAP gene.